@*%#$@$%^&!!!!! basically sums up my morning thus far.
The past two days, I've been sidelined with a miserable migraine. The vomiting, loss-of-vision, bang-your-head-against-the-wall variety. Today, I finally dragged myself out of bed and into lab because I know my proteins have a very short shelf life, and I need to get an IP done before one or the other degraded. I figured maybe my reward would finally be getting something to work.
As I sit here, miserable, pounding headache, dizzy from the Midrin, not quite seeing straight, I was feeling comfortable with the labwork -- I've done IP's a million times to the point that I can do them in my sleep (quite literally -- I've been dreaming about them quite a bit lately). And since these proteins degrade within a 5-6 day span, this needed to be done. So, here I am.
But just now, I realize, that like a COMPLETE $*#@ing non-competent moron, rather than an ammonium sulfate precipitation, I apparently attempted to precipitate my proteins with ammonium PERSULFATE this morning.
I drag myself out of bed with a migraine to try and complete an experiment to please my PI, and wind up wasting all the protein I have. Screw this. I am never getting out of bed again.
The past two days, I've been sidelined with a miserable migraine. The vomiting, loss-of-vision, bang-your-head-against-the-wall variety. Today, I finally dragged myself out of bed and into lab because I know my proteins have a very short shelf life, and I need to get an IP done before one or the other degraded. I figured maybe my reward would finally be getting something to work.
As I sit here, miserable, pounding headache, dizzy from the Midrin, not quite seeing straight, I was feeling comfortable with the labwork -- I've done IP's a million times to the point that I can do them in my sleep (quite literally -- I've been dreaming about them quite a bit lately). And since these proteins degrade within a 5-6 day span, this needed to be done. So, here I am.
But just now, I realize, that like a COMPLETE $*#@ing non-competent moron, rather than an ammonium sulfate precipitation, I apparently attempted to precipitate my proteins with ammonium PERSULFATE this morning.
I drag myself out of bed with a migraine to try and complete an experiment to please my PI, and wind up wasting all the protein I have. Screw this. I am never getting out of bed again.