Adieu Amber Ale... So long, farewell, Hefeweizen! Bye-bye Bud Light...
The last wheat products to go...
My phytoestrogens will have to be sourced elsewhere like from barley - only beer -- GO HOP -- **hip-hop** HURRAY...
See below abstract -- Luv our microscopic friends and fauna that reside in our mile-long bowels! Treat them well...
In my former career as a research associate, I worked on barley and calmodulin (yes a target of Vitamin D -- who knew?). A great deal of my time was spent on northern blots (which takes a certain knack b/c RNA deteriorates easily) and southerns (DNA's durable Thank God). Yes, we were partially funded by the USDA (as well as beer companies.... j/k! maybe ??indirectly??). Had to test theories and research qualitative factors... after work beer with the post-docs and grad students was often a job requirement... no joke *hee*.
Activation of proestrogens from hops (Humulus lupulus L.) by intestinal microbiota; conversion of isoxanthohumol into 8-prenylnaringenin.
Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Gent University-UGent, Coupure 653, Gent, Belgium.
Hop , an essential ingredient in most beers, contains a number of prenylflavonoids, among which 8-prenylnaringenin (8-PN) would be the most potent phytoestrogen currently known. Although a number of health effects are attributed to these compounds, only a few reports are available about the bioavailability of prenylflavonoids and the transformation potency of the intestinal microbial community . To test these transformations, four fecal samples were incubated with xanthohumol, isoxanthohumol (IX), and 8-PN. Upon incubation with IX, present in strong ales up to 4 mg/L, 36% was converted into 8-PN in one fecal sample and the estrogenic properties of the sample drastically increased. In an experiment with 12 fecal cultures, this conversion was observed in one-third of the samples, indicating the importance of interindividual variability in the intestinal microbial community. Eubacterium limosum was identified to be capable of this conversion (O-demethylation) of IX into 8-PN, and after strain selection, a conversion efficiency of 90% was achieved. Finally, strain supplementation to a nonconverting fecal sample led to rapid and high 8-PN production at only 1% (v/v) addition. Up to now, the concentration of 8-PN in beer was considered too low to affect human health. However, these results show that the activity of the intestinal microbial community could more than 10-fold increase the exposure concentration. Because prenylflavonoids are present in many beers with IX being the major constituent, the results raise the question whether moderate beer consumption might contribute to increased in vivo levels of 8-PN and even influence human health.
PMID: 16076107
Stuff from my old lab below -- flora and fauna -- sometimes not much different. Many hormone actions and metabolic rules are conserved across nature.
Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers. Beligni MV, et al. Plant Physiol. 2002 Aug;129(4):1642-50.
Hormonally regulated programmed cell death in barley aleurone cells. Bethke PC, et al. Plant Cell. 1999 Jun;11(6):1033-46.
By Shwayze
Courtesy of Youtube.com
Will miss you... RIP
Adieu Amber Ale... So long, farewell, Hefeweizen! Bye-bye Bud Light...
The last wheat products to go...
My phytoestrogens will have to be sourced elsewhere like from barley - only beer -- GO HOP -- **hip-hop** HURRAY...
See below abstract -- Luv our microscopic friends and fauna that reside in our mile-long bowels! Treat them well...
In my former career as a research associate, I worked on barley and calmodulin (yes a target of Vitamin D -- who knew?). A great deal of my time was spent on northern blots (which takes a certain knack b/c RNA deteriorates easily) and southerns (DNA's durable Thank God). Yes, we were partially funded by the USDA (as well as beer companies.... j/k! maybe ??indirectly??). Had to test theories and research qualitative factors... after work beer with the post-docs and grad students was often a job requirement... no joke *hee*.
- Activation of proestrogens from hops (Humulus lupulus L.) by intestinal microbiota; conversion of isoxanthohumol into 8-prenylnaringenin.
Stuff from my old lab below -- flora and fauna -- sometimes not much different. Many hormone actions and metabolic rules are conserved across nature.Possemiers S, et al.
J Agric Food Chem. 2005 Aug 10;53(16):6281-8.
Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Gent University-UGent, Coupure 653, Gent, Belgium.
Hop , an essential ingredient in most beers, contains a number of prenylflavonoids, among which 8-prenylnaringenin (8-PN) would be the most potent phytoestrogen currently known. Although a number of health effects are attributed to these compounds, only a few reports are available about the bioavailability of prenylflavonoids and the transformation potency of the intestinal microbial community . To test these transformations, four fecal samples were incubated with xanthohumol, isoxanthohumol (IX), and 8-PN. Upon incubation with IX, present in strong ales up to 4 mg/L, 36% was converted into 8-PN in one fecal sample and the estrogenic properties of the sample drastically increased. In an experiment with 12 fecal cultures, this conversion was observed in one-third of the samples, indicating the importance of interindividual variability in the intestinal microbial community. Eubacterium limosum was identified to be capable of this conversion (O-demethylation) of IX into 8-PN, and after strain selection, a conversion efficiency of 90% was achieved. Finally, strain supplementation to a nonconverting fecal sample led to rapid and high 8-PN production at only 1% (v/v) addition. Up to now, the concentration of 8-PN in beer was considered too low to affect human health. However, these results show that the activity of the intestinal microbial community could more than 10-fold increase the exposure concentration. Because prenylflavonoids are present in many beers with IX being the major constituent, the results raise the question whether moderate beer consumption might contribute to increased in vivo levels of 8-PN and even influence human health.
PMID: 16076107