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Nucleic Acid Amplification Technique for Rapid Diagnostic Analysis

Posted Aug 12 2013 8:00pm

Description of Invention:
CDC researchers developed a simple target-specific isothermal nucleic acid amplification technique, termed Genome Exponential Amplification Reaction (GEAR). The method employs a set of four primers (two inner and two outer). The outer primer pair targets three specific nucleic acid sequences at a constant 60°C, while the inner pair of primers accelerates and improves the sensitivity of the assay. The GEAR technique is an improvement over loop-mediated isothermal amplification (LAMP) in three ways. First, the GEAR method uses two Tab primers which target three genomic regions (corresponding LAMP primers target four regions). Second, the GEAR method features complementary 5' ends between the forward and reverse primers. Third, the GEAR method does not require a second set of outer primers (LAMP requires two outermost primers). Additionally, the GEAR isothermal method can be performed in a relatively inexpensive water bath or heating block, with detection of amplification products by fluorescence, thus making it suitable for low resource settings.

Applications:
  • Rapid diagnostic analysis of biological samples
  • Qualitative and quantitative analysis of nucleic acids
  • Low-cost diagnostics for malaria, tuberculosis, and other infectious diseases


Advantages:
  • Rapid, portable, cost-effective
  • Useful in low resource settings
  • A “single-tube” assay that eliminates need for thermal cyclers or gel electrophoresis
  • Unlike many other isothermal amplification approaches, GEAR can be efficiently performed at temperatures exceeding 60 °C, increasing specificity and accuracy


Development Status:
  • Pre-clinical
  • In vitro data available


Inventors:
Jothikumar Narayanan (CDC Confirmed not Georgia Inst of Tech.)
Prithiviraj Jothikumar (CDC)
Vincent R Hill (CDC)


Patent Status:
HHS, Reference No. E-273-2013/0
PCT, Application No. PCT/US2012/049784 filed 06 Aug 2012


Relevant Publication:
  1. Prithiviraj J, et al. [ PMID 22450319 ]



For Licensing Information Please Contact:
Whitney Blair
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: blairw2@mail.nih.gov
Phone: 301-435-4937
Fax: 301-402-0220


Ref No: 2615

Updated: 08/2013

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