Nucleic Acid Amplification Technique for Rapid Diagnostic Analysis
Posted Aug 12 2013 8:00pm
Description of Invention: CDC researchers developed a simple target-specific isothermal nucleic acid amplification technique, termed Genome Exponential Amplification Reaction (GEAR). The method employs a set of four primers (two inner and two outer). The outer primer pair targets three specific nucleic acid sequences at a constant 60°C, while the inner pair of primers accelerates and improves the sensitivity of the assay.
The GEAR technique is an improvement over loop-mediated isothermal amplification (LAMP) in three ways. First, the GEAR method uses two Tab primers which target three genomic regions (corresponding LAMP primers target four regions). Second, the GEAR method features complementary 5' ends between the forward and reverse primers. Third, the GEAR method does not require a second set of outer primers (LAMP requires two outermost primers). Additionally, the GEAR isothermal method can be performed in a relatively inexpensive water bath or heating block, with detection of amplification products by fluorescence, thus making it suitable for low resource settings.
Rapid diagnostic analysis of biological samples
Qualitative and quantitative analysis of nucleic acids
Low-cost diagnostics for malaria, tuberculosis, and other infectious diseases
Rapid, portable, cost-effective
Useful in low resource settings
A “single-tube” assay that eliminates need for thermal cyclers or gel electrophoresis
Unlike many other isothermal amplification approaches, GEAR can be efficiently performed at temperatures exceeding 60 °C, increasing specificity and accuracy
In vitro data available
Inventors: Jothikumar Narayanan (CDC Confirmed not Georgia Inst of Tech.) Prithiviraj Jothikumar (CDC) Vincent R Hill (CDC)
For Licensing Information Please Contact: Whitney Blair NIH Office of Technology Transfer 6011 Executive Blvd. Suite 325, Rockville, MD 20852 United States Email: email@example.com Phone: 301-435-4937 Fax: 301-402-0220