Molecular Probes for Identification or Isolation of Membrane Proteins

Description of Invention:
This technology describes a new class of molecular probes designed around an iodonaphthyl succinate antigen that can be used to label and tag proteins using a variety of conventional protein modification chemistries. The technology is offered as a combination of probe + monoclonal antibodies against the probe (three clones). The probe can be used for labeling and tagging cell surface and integral membrane proteins as well as soluble proteins. The monoclonal antibodies were tested and found effective for immunoprecipitation, western blot, and flow cytometry. Once tagged, the modified proteins can be detected or isolated using an antibody reactive with the probe. Several possible probes and monoclonal antibodies that react with them are described. These probes and their corresponding antibodies have significant advantages over the biotin-avidin system.

Applications:

• Protein labeling
• Protein isolation

Advantages:

• Reversibility of binding for protein isolation
• Lack of high, non-specific binding to cell surfaces
• Ability to incorporate isotopic ¹²⁵I label in the probe for tracking tagged proteins in vivo

Development Status:
In vitro data available

Inventors:
Yossef Raviv (NCI)



Portfolios:
Devices/Instrumentation
Devices/Instrumentation - Research Tools and Materials
In-vitro Data



For Additional Information Please Contact:
Kevin Chang Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: changke@mail.nih.gov
Phone: 301-435-5018
Fax: 301-402-0220


Ref No: 1590

Updated: 06/2010