Composition and Methods for Rapid Detection of HIV by Loop-mediated Isothermal Amplification (LAMP)
Posted Aug 22 2013 8:00pm
Description of Invention: This invention relates to methods and compositions for rapid detection of HIV nucleic acids in a biological sample. Specifically, it involves the use of the loop-mediated isothermal amplification (LAMP) for rapid detection of HIV-1 and/or HIV-2. The use of rapid HIV tests is highly attractive for screening of patient samples, especially in developing countries where resources are limited, because they are quick, easy to perform, and do not require any special equipment. Rapid tests for the identification of HIV antibody, however, will remain negative during the 4 to 5 week window post-infection and pre-seroconversion, necessitating the need for a diagnosis based on HIV nucleic acid.
Diagnostic test for HIV-1 and/or HIV-2 infection
Kits for detection of HIV nucleic acids
High sensitivity and specificity.
No need for thermal cyclers or gel electrophoresis; assay can be used in limited-resource settings.
A rapid, portable and cost-effective alternative to PCR and enzyme immune assays (EIA).
In vitro data available
Inventors: Michele S Owen (CDC) Kelly Curtis (CDC) Donna L Rudolph (CDC)
For Licensing Information Please Contact: Whitney Blair NIH Office of Technology Transfer 6011 Executive Blvd. Suite 325, Rockville, MD 20852 United States Email: email@example.com Phone: 301-435-4937 Fax: 301-402-0220