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CDK4-Transformed Mouse Podocytes Useful for Studying Glomerular Diseases

Posted Jul 26 2011 8:00pm

Description of Invention:
Podocytes, cells of the visceral epithelium in the kidneys, are a key component of the glomerular filtration barrier. Podocyte damage and loss contribute to the initiation of glomerular diseases. Cyclin-dependent kinase 4 (CDK4), a catalytic subunit of the cyclin D-CDK4 serine/threonine kinase complex, is a critical regulator of the cell cycle. Recent studies showed that cells immortalized with CDK4 are useful to study pathophysiology. NIH investigators have generated mouse podocytes transformed with CDK4 as a nonviral immortalizing gene. These transformed podocytes show podocyte characteristics and express podocyte markers. Furthermore, confluent CDK4-podocyte cultures show higher levels of gene expression for multiple podocyte differentiation genes compared with subconfluent or lower density culture.

Applications:
  • Model system for study of glomerular disorders.
  • Useful tools to study podocyte biology.


Advantages:
Better model system to study podocyte structure and function.

Development Status:
  • Early-stage
  • Pre-clinical
  • In vitro data available


Inventors:
Jeffrey B Kopp (NIDDK)
Toru Sakairi (NIDDK)


Patent Status:
HHS, Reference No. E-287-2010/0

Research Tool (Materials available for licensing: CDK4 podocytes) — Patent protection is not being pursued for this technology.

Related Technologies:
EIR, Reference No. E-049-2007/0


Research Tool — Model for Study of Glomerular Disorders: Conditionally-Immortalized Mouse Podocyte Cell Line with Tet-on-Regulated Gene Expression (Dr. Jefferey B. Kopp, NIDDK)

Relevant Publication:
  1. Sakairi T, et al. [ PMID 20668098 ]



For Licensing Information Please Contact:
Suryanarayana Vepa Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: vepas@mail.nih.gov
Phone: 301-435-5020
Fax: 301-402-0220


Ref No: 2293

Updated: 07/2011

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