Description of Invention: Available for licensing and commercial development are intellectual property rights covering a method of imaging a biological specimen (e.g., human tissue) using fluorescent nanodiamonds implanted into the subject of interest, applying a magnetic field to said subject and producing a resultant image by a net juxtaposition of a second acquired image. This process suppresses the background and permits selective imaging of the nanodiamonds in the presence of background fluorescence that exceeds the signal from the nanodiamonds. Another aspect of the invention provides an imaging method in which the resulting image is acquired by applying time-varying magnetic fields using one or more secondary image averaged against the first. The technique relies on imposing a small (~100 Gauss) magnetic field on the sample of interest during optical imaging combined with post-processing of the acquired images to remove the background. This technology can readily be added onto any commercial optical imaging platform to achieve background-free images of the nanodiamonds in a biological specimen.
In vitro and in vivo optical imaging and diagnostics
Improved resolution through composite imagery
Indefinite tracking due to the exceptional stability of the fluorescent nanodiamonds
Wide excitation band (~500-600 nm)
Broad-band Near IR emission (600-700 nm)
Nanodiamonds are stable in aqueous solution
In related technologies we have developed a method to specifically coat and functionalize nanodiamonds for targeting and labeling applications
In vitro data available
In vivo data available (animal)
Inventors: Ambika Bumb (NHLBI) Susanta K Sarkar (NHLBI) Keir C Neuman (NHLBI)
Collaborative Research Opportunity: The NHLBI Laboratory of Single Molecule Biophysics is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize background-free imaging of fluorescent nanodiamonds for in vivo and in vitro applications. For collaboration opportunities, please contact Keir C. Neuman, Ph.D. at email@example.com or 301-496-3376.
For Licensing Information Please Contact: Michael Shmilovich Esq. NIH Office of Technology Transfer 6011 Executive Blvd. Suite 325, Rockville, MD 20852 United States Email: firstname.lastname@example.org Phone: 301-435-5019 Fax: 301-402-0220