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Background-Free Fluorescent Nanodiamond Imaging

Posted Feb 20 2013 7:00pm

Description of Invention:
Available for licensing and commercial development are intellectual property rights covering a method of imaging a biological specimen (e.g., human tissue) using fluorescent nanodiamonds implanted into the subject of interest, applying a magnetic field to said subject and producing a resultant image by a net juxtaposition of a second acquired image. This process suppresses the background and permits selective imaging of the nanodiamonds in the presence of background fluorescence that exceeds the signal from the nanodiamonds. Another aspect of the invention provides an imaging method in which the resulting image is acquired by applying time-varying magnetic fields using one or more secondary image averaged against the first. The technique relies on imposing a small (~100 Gauss) magnetic field on the sample of interest during optical imaging combined with post-processing of the acquired images to remove the background. This technology can readily be added onto any commercial optical imaging platform to achieve background-free images of the nanodiamonds in a biological specimen.

  • In vitro and in vivo optical imaging and diagnostics
  • MRI imaging

  • Improved resolution through composite imagery
  • Background elimination
  • Indefinite tracking due to the exceptional stability of the fluorescent nanodiamonds
  • Wide excitation band (~500-600 nm)
  • Broad-band Near IR emission (600-700 nm)
  • Nanodiamonds are stable in aqueous solution
  • In related technologies we have developed a method to specifically coat and functionalize nanodiamonds for targeting and labeling applications

Development Status:
  • Prototype
  • In vitro data available
  • In vivo data available (animal)

Ambika Bumb (NHLBI)
Susanta K Sarkar (NHLBI)
Keir C Neuman (NHLBI)

Patent Status:
HHS, Reference No. E-261-2012/0
US, Application No. 61/711,702 filed 09 Oct 2012

Related Technologies:
US, Application No. 61/672,996 filed 18 Jul 2012, Reference No. E-175-2012/0

Collaborative Research Opportunity:
The NHLBI Laboratory of Single Molecule Biophysics is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize background-free imaging of fluorescent nanodiamonds for in vivo and in vitro applications. For collaboration opportunities, please contact Keir C. Neuman, Ph.D. at or 301-496-3376.

For Licensing Information Please Contact:
Michael Shmilovich Esq.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Phone: 301-435-5019
Fax: 301-402-0220

Ref No: 2532

Updated: 02/2013

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