Multiplex PCR microarray assay screens multiple bioterror pathogens in blood
Posted Sep 12 2008 6:59pm
According to a study from the United States, “Heightened concern about the dangers of bioterrorism requires that measures be developed to ensure the safety of the blood supply. Multiplex detection of such agents using a blood-screening DNA microarray is a sensitive and specific method to screen simultaneously for a number of suspected agents.”
“We have developed and optimized a multiplex polymerase chain reaction microarray assay to screen blood for three potential bioterror bacterial pathogens and a human ribosomal RNA gene internal control,” said K. Tomioka and colleagues at the U.S. National Institutes of Health.
“The analytical sensitivity of the assay was demonstrated to be 50 colony-forming units/mL for Bacillus anthracis, Francisella tularensis, and Yersinia pseudotuberculosis (surrogate for Yersinia pestis). The absence of any false-positives demonstrated high analytical specificity,” scientists repor