Health knowledge made personal
Join this community!
› Share page:
Go
Search posts:

Low Level Laser Medicine of the Future, Part One

Posted Dec 02 2011 4:11pm
Three lasers projected into spaceLow Level Laser Medicine of the Future, Part One

I recently learned about Low Level Laser  at a meeting in Orlando.  Dr. Larry Lytle presented a nice talk on the low energy laser device for various medical applications.  This information was new to me as it probably is for you as well. 

Left Image: Sky with laser beams, with three lasers beams projected into the sky courtesy of Wikimedia Commons.

A Patient with Severe Degenerative Arthritis

One of out family members has severe degenerative arthritis of the knee, so we picked up a low level laser device from Dr Lytle with the idea of using it on the knee. .  MRI scans showed advanced degenerative changes, destruction of knee cartilage, and fluid in the joint, and the knee was inflamed, and painful. 

QLaser Device Courtesy of Dr Lytle and QLaserLeft Image: QLaser Device Courtesy of Dr Lytle and QLaser Company

Offering a
Knee Replacement

The knee was bad enough for the orthopedic surgeon to offer a knee replacement. 

Low Level Laser for the Knee

We applied the  low level laser light to the knee, and noticed prompt improvement with less pain and inflammation.  With daily treatments, the knee continued to improve with elimination of pain.  The knee replacement operation is no longer needed.


What is Low Level Laser?   And Why Haven't I Heard About It?

You are probably thinking, "if low level laser is so great, why haven't I heard about it"?  The answer is that the FDA, working on behalf of the drug industry, has been diligently suppressing information about it.  The device relieves pain and inflammation directly competing with the drug industry.  Dr Lytle received FDA warning letters demanding removal of information from his web site  about uses of the low level laser.  Fortunately, this newsletter can fill in the missing information deleted by the FDA.

What Are Low Level Lasers ?

Invented in 1967, LASER stands for Light Amplification and Stimulated Emission.  This is a device which produces light which is In-Phase and all the Same Frequency, also called Coherent Light.  This differs from incoherent light from the sun or a light bulb, this type of light is out of phase and has differing frequencies.  

Since invention in 1967, lasers have become commonplace in medical, industrial and even household uses.  For example, there are low level lasers in your CD player, in the supermarket barcode reader, and in your optical mouse. 

Low level lasers for personal medical use are also available, approved as safe by the FDA, and available for sale to the public without a prescription.  These can be seen in popular mail order catalogs, and can even be ordered from Amazon.

Of course, we make the important distinction between safe low energy lasers and the more dangerous high energy lasers which can cut through metal. Medical  lasers are used by surgeons to cut tissue in the operating room.  High Energy Lasers are obviously dangerous and require more regulation.

Energy- Where Does It Come From ?  Photosynthesis from Light

All energy in living organisms comes from light energy (from the sun) harnessed by photosynthesis.  In higher organisms, such as us humans, we eat the plants and utilize plant energy in the form of carbon double bonds in a reaction called oxidation.  This same oxidation provides us with energy from "fossil fuel" such as coal, oil and gasoline which are another source of energy stored up from plant photosynthesis. An "oxidative" chemical reaction occurs when we use fossil fuel, and "burn" a log in the fire place, or run our car engine with gasoline.

The Proto-Porphyrin Ring- This Absorbs Light Energy

Proto-porphyrin Basic Ring Structure
Left Image: Proto-porphyrin Basic Ring Structure - notice the four central nitrogen atoms. This structure absorbs light and is present as hemoglobin, chlorophyll, and all cytochromes.  Central metal atoms such as Fe , Mg and Cu enhance ability to absorb light energy.

How Our Cells Make Energy

Normally, the mitochondria in trillions of our cells use food to make energy.  Food is the carbon compounds we eat.  The carbon double bond is the energy source and provides energy in a process called oxidation (also called Fatty Acid Oxidation or oxidative phosphorylation).  Oxygen is combined with carbon (from our food) to liberate the energy in the double bond in "burning" of carbon  to carbon dioxide and water. 

Photosynthesis in Mitochondria ?

Another dormant pathway of energy production also exists.  Our mitochondria retain the ability to absorb energy from light.  The more primitive machinery of "photosynthesis" lies dormant in mitochondria, ready to be re-awakened. The  mitochondria can absorb and utilize energy directly from light photons in the infrared spectrum.  This machinery is very similar to photosynthesis of plants and bacteria.

Living Organisms Absorb and Utilize  Light Energy - Photosynthesis


Chlorophyll found in bacteria
Left Image: Chlorophyll found in bacteria.  Notice the Proto-Porphyrin Ring with central (Mg) Magnesium, surrounded by four nitrogen atoms.  This structure absorbs light energy.

 



We are all familiar with photosynthesis, the ability of plants to convert sunlight into usable energy.  The leafy green color comes from chlorophyll, the light absorptive molecule.  The chemical structure of chlorophyll is strikingly similar to hemoglobin, both contain a proto-porphyrin ring, and a central metal atom.  Chlorophyll contains magnesium (Mg) in the center.  Hemoglobin contains iron (Fe), and Cytochrome C oxidase contains both Fe  and Copper.  Mitochondria contain light absorbing compounds called cytochromes which contain the same proto-porphyrin ring structure as chlorophyll, allowing for light absorption . (see image below)

Cytochrome C Oxidase in the mitochondriaLeft Image : Cytochrome C Oxidase in the mitochondria.  Notice similarity of ring structure to chlorophyll.  Central Magnesium has been replace with a central Iron (Fe).  There is also a copper (Cu) add-on at top.  This structure absorbs light energy.

Where in the cell is the light absorbed ?  Cytochrome C Oxidase

Recent studies show that light photons are absorbed in the mitochondria by a protein called cytochrome c oxidase, which is involved in energy production.  Cytochrome c oxidase is a photo-acceptor, absorbing light at a peak spectrum of 630-670nm (red light).  Cytochrome C oxidase is a large protein which contains two Iron molecules and two Copper molecules in its center .   These metals atoms have outer shell electrons which absorb electromagnetic radiation (also known as a light photons). 



Activating Cytochrome C Oxidase in the Mitochondria

Mitochonria with inner membrane showing electrical energy production.Left Image: Mitochondria with illuminated inner membrane showing electrical energy production.

Once the outer electrons of the central metal atom absorb light energy, these electrons will then move to higher-energy orbitals, making a transition to a higher-energy state.  This is useful since the job of Cytochrome Oxidase is energy production and the increased energy spills out in the final product as ATP. 

Thus we have a process of converting light energy to usable cellular ATP (Adenine Triphosphate) which is the currency of energy in the cell.  This shares remarkable molecular similarities to photosynthesis in plants and bacteria. 

Thanks to low level laser devices, we can now deliver photon energy to mitochondria of deeper areas of the body such as joints, the spine and brain.   This "energizes" the mitochondria, and increased cellular energy production activates the healing process.

Gene Expression and Cell Signalling

However, increased mitochondria energy is only where the process begins.  This is only the first step which triggers cell signalling for growth and healing.  In addition, there is increased microcirculation from nitric oxide synthesis, there is activation of fibroblasts and macrophages.  There is stimulation of new blood vessel growth. There is up-regulation of hundred of genes which are involved in transcription of growth and proliferation factors such as nuclear factor kappa beta, VEGF (vascular endothelial growth factor), and the JNK gene is activated as well with promoted phosphorylation of Jun N-terminal kinase (JNK)/activator protein-1 (AP-1) expressions.  Bottom line is this induces a healing reaction.

For Part Two of this series, CLICK HERE .


Jeffrey Dach MD
7450 Griffin Road, SUite 190
Davie, Fl 33314
954-792-4663
www.jeffreydach.com
www.drdach.com
www.naturalmedicine101.com
www.bioidenticalhormones101.com
www.truemedmd.com


Links and References

http://pubs.acs.org/cen/news/85/i12/8512notw4.html
Chemical & Engineering News. March 19, 2007     Volume 85, Number 12    p. 13
Enzyme Catalysis Electron-Starved Enzyme Cytochrome c oxidase model mimics natural electron-limited conditions SChematic model of cytochrome C oxidase

http://mail.sott.net/articles/ show/223040-Extinction- Metamorphosis-Evolutionary- Apoptosis-and-Genetically- Programmed-Species-Mass-Death

 Extinction, Metamorphosis, Evolutionary Apoptosis, and Genetically Programmed Species Mass Death Rhawn Joseph, Ph.D. Journal of Cosmology Thu, 15 Oct 2009 07:58 CDT


http://mail.sott.net/articles/ show/223040-Extinction- Metamorphosis-Evolutionary- Apoptosis-and-Genetically- Programmed-Species-Mass-Death

 Oxygenation and Mitochondria



---------------------- Literature review -------------------------

www.laserscenarfusion.com/articles/newlllt.pdf

Our latest LLLT literature review with abstracts including muscle fatigue, TMJ, myofacial pain, acne and a possible application for morphine withdrawal. selected pubmed references from this pdf file:

---------------
increases ATP in lymphocytes
----------------

www.ncbi.nlm.nih.gov/pubmed/18922088

Photomed Laser Surg. 2008 Oct;26(5):451-3.

Intracellular ATP level increases in lymphocytes irradiated with infrared laser light of wavelength 904 nm. Benedicenti S, Pepe IM, Angiero F, Benedicenti A. Source Department of Medical Science, Dentistry, and Biophysics, University of Genoa, Milan, Italy. stefano.benediceneti@tiscali.it Abstract

OBJECTIVE: Red and near-infrared laser irradiation is reported to have a range of biological effects on cultured cells and different tissues, leading to the hypothesis that laser light can affect energy metabolism. Increased adenosine triphosphate (ATP) synthesis has been reported in cultured cells and rat brain tissue after irradiation at 632.8 nm and 830 nm, respectively. This study investigated whether diode pulsed laser irradiation enhances ATP production in lymphocytes.

MATERIALS AND METHODS: Aliquots (500 microL) of an extract of cultured lymphocytes of the Molt-4 cell line were irradiated with diode laser light (lambda = 904 nm, pulsed mode, 6 kHz frequency) with an average emission power of 10 mW for 60 min. A Spectra Physics M404 power meter was used to measure light intensity. Controls were treated similarly but not irradiated. The amount of ATP was measured by the luciferin-luciferase bioluminescent assay. RESULTS: The amount of ATP in irradiated cell cultures was 10.79 +/- 0.15 microg/L (SD; n = 10), and in non-irradiated cell cultures it was 8.81 +/- 0.13 microg/L (SD; n = 10).

The average percentage increase of irradiated versus control cell cultures was about 22.4% +/- 0.56% SD (p < 0.001).

CONCLUSION: This significant increase is probably due to laser irradiation; it cannot be attributed to any thermal effect, as the temperature during irradiation was maintained at 37.0 degrees +/- 0.5 degrees C. Thus the therapeutic effects of the biostimulating power of this type of laser are identified and its indications may be expanded. Increases nitric oxide

www.ncbi.nlm.nih.gov/pubmed/18922087?dopt=Citation

Photomed Laser Surg. 2008 Oct;26(5):443-9. Role of nitric oxide in the visible light-induced rapid increase of human skin microcirculation at the local and systemic levels: II. healthy volunteers. Samoilova KA, Zhevago NA, Petrishchev NN, Zimin AA. Source Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. samoilova3@yandex.ru Abstract

OBJECTIVE: The aim of this study is to evaluate the skin microcirculation increase seen in healthy volunteers after a single exposure to polychromatic visible (pVIS) light, and to prove the role of nitric oxide (NO) in the development of this effect.

BACKGROUND DATA: Improvement of microcirculation is one of the most important effects of laser and pVIS light therapy; however, its mechanism of action remains unknown. A main role in the regulation of vascular tone is known to be played by NO. It is produced by NO-synthase (NOS) located in membranes of many cells, including endothelial and blood cells. NOS, a biopteroflavohemoprotein, absorbs pVIS light, resulting in its activation.

MATERIALS AND METHODS: The central area of the dorsal side of the right hand (24 cm2) of 42 volunteers was irradiated for 5 min with pVIS light from a Q-light (385-750 nm, 95% polarization, 40 mW/cm2, 12 J/cm2). Then for 90 min, the blood flow rate (Qas) was measured eight times, both in the area of the irradiation (local effect) and in the non-irradiated left hand (systemic effect) by using a high-frequency ultrasound Doppler device, recording Qas in human skin to a depth up to 5 mm. In the central area of the right hand of 14 volunteers an NOS inhibitor, N-monomethyl-L-arginine (L-NMMA, 0.1% solution), was iontophoretically administered prior to exposure, whereas in 10 other subjects it was administered to the left hand with subsequent exposure of the right hand.

RESULTS: As soon as 2 min after exposure, Qas in the irradiated area rose on average by 32%, and in 20 min by 45%; it then decreased and in 90 min returned to the initial level. A statistically significant Qas increase in the non-irradiated hand was recorded in 5 min (+9%), and in 20 min it reached a maximum level (+39%), and 90 min later it decreased to the initial values. The presence of L-NMMA in the light-exposed area completely blocked the photoinduced rise of microcirculation, both in the irradiated and in non-irradiated hand; however, its administration to the non-irradiated hand did not prevent these effects.

CONCLUSION: The increase in skin microcirculation produced by pVIS light at the local and systemic levels is due to activation of NO synthesis in the irradiated area.

-------
full text Free Mechanism of Benefits of Low Level Lasers-
Activates Mitochondrial respiration-

www.ncbi.nlm.nih.gov/pubmed/21814580
PLoS One. 2011;6(7):e22453. Epub 2011 Jul 21.

Low-level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts.

Chen AC, Arany PR, Huang YY, Tomkinson EM, Sharma SK, Kharkwal GB, Saleem T, Mooney D, Yull FE, Blackwell TS, Hamblin MR. Source Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America. BACKGROUND: Despite over forty years of investigation on low-level light therapy (LLLT), the fundamental mechanisms underlying photobiomodulation at a cellular level remain unclear.

METHODOLOGY/PRINCIPAL FINDINGS: In this study, we isolated murine embryonic fibroblasts (MEF) from transgenic NF-kB luciferase reporter mice and studied their response to 810 nm laser radiation. Significant activation of NF-kB was observed at fluences higher than 0.003 J/cm(2) and was confirmed by Western blot analysis. NF-kB was activated earlier (1 hour) by LLLT compared to conventional lipopolysaccharide treatment. We also observed that LLLT induced intracellular reactive oxygen species (ROS) production similar to mitochondrial inhibitors, such as antimycin A, rotenone and paraquat. Furthermore, we observed similar NF-kB activation with these mitochondrial inhibitors. These results, together with inhibition of laser induced NF-kB activation by antioxidants, suggests that ROS play an important role in the laser induced NF-kB signaling pathways. However, LLLT, unlike mitochondrial inhibitors, induced increased cellular ATP levels, which indicates that LLLT also upregulates mitochondrial respiration.

CONCLUSION: We conclude that LLLT not only enhances mitochondrial respiration, but also activates the redox-sensitive NFkB signaling via generation of ROS. Expression of anti-apoptosis and pro-survival genes responsive to NFkB could explain many clinical effects of LLLT.
--------------------------------------
FUll text- nice intro and review by Harvard prof
--------------------------------

www.ncbi.nlm.nih.gov/pmc/articles/PMC2939452/?tool=pubmed

Lasers Surg Med. 2010 August; 42(6): 447–449.
Introduction to Experimental and Clinical Studies Using Low-Level Laser (Light) Therapy (LLLT)

Michael R. Hamblin, PhD, Associate Professor Michael R. Hamblin, Harvard Medical School; *Correspondence to: Michael R Hamblin, PhD, Assistant Professor, Harvard Medical School

-------------------
Photoceutical for stem cell growth and regeneration
 -----------------------------

From a Stem Cell COmpany:

www.ncbi.nlm.nih.gov/pmc/articles/PMC2830167/?tool=pubmed

J Transl Med. 2010; 8: 16.
Lasers, stem cells, and COPD
Feng Lin,#1 Steven F Josephs,#1 Doru T Alexandrescu,#2 Famela Ramos,1 Vladimir Bogin,3 Vincent Gammill,4 Constantin A Dasanu,5 Rosalia De Necochea-Campion,6 Amit N Patel,7 Ewa Carrier,6 and David R Kooscorresponding author1

The medical use of low level laser (LLL) irradiation has been occurring for decades, primarily in the area of tissue healing and inflammatory conditions. Despite little mechanistic knowledge, the concept of a non-invasive, non-thermal intervention that has the potential to modulate regenerative processes is worthy of attention when searching for novel methods of augmenting stem cell-based therapies. Here we discuss the use of LLL irradiation as a "photoceutical" for enhancing production of stem cell growth/chemoattractant factors, stimulation of angiogenesis, and directly augmenting proliferation of stem cells. The combination of LLL together with allogeneic and autologous stem cells, as well as post-mobilization directing of stem cells will be discussed. "A pubmed search for "low level laser therapy" yields more than 1700 results, yet before stumbling across this concept, none of us, or our advisors, have ever heard of this area of medicine."

 --------------------------- Nuclear Factor Kappa Beta -------------------------- www.researchersworld.com/vol2/issue3/Paper_6.pdf

THE BIOLOGY OF NUCLEAR FACTOR KAPPA BETA (NFkB IN HEALTH AND PATHOLOGY by Carlos Kusano Bucalen Ferrari, Biomedical Research Group, Institute of Biological and Health Sciences (ICBS), “Campus Universitário do Araguaia”, “Universidade Federal de Mato Grosso” (UFMT), Barra do Garças, MT, Brazil.


www.ncbi.nlm.nih.gov/pubmed/15175863
J Mol Med (Berl). 2004 Jul;82(7):434-48. Epub 2004 Jun 3.
Nuclear factor-kappaB: its role in health and disease.
Kumar A, Takada Y, Boriek AM, Aggarwal BB. Source Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA. axkumar@bcm.tmc.edu Abstract Nuclear factor-kappaB (NF-kappa is a major transcription factor that plays an essential role in several aspects of human health including the development of innate and adaptive immunity. The dysregulation of NF-kappaB is associated with many disease states such as AIDS, atherosclerosis, asthma, arthritis, cancer, diabetes, inflammatory bowel disease, muscular dystrophy, stroke, and viral infections. Recent evidence also suggests that the dysfunction of NF-kappaB is a major mediator of some human genetic disorders. Appropriate regulation and control of NF-kappaB activity, which can be achieved by gene modification or pharmacological strategies, would provide a potential approach for the management of NF-kappaB related human diseases. This review summarizes the current knowledge of the physiological and pathophysiological functions of NF-kappaB and its possible role as a target of therapeutic intervention

-----------------------------------------------------------------

www.drkaslow.com/html/low_level_lasers__lllt_.html

cold laser article dr kaslow place into blog roll

---------- Q LAser dr Lytle _----------------

 “Use Mode 1 to re-energize muscle, ligament and tendon cells for healing wounds and injuries or for reducing pain and inflammation; Mode 1 also benefits tendonitis, arthritis, burns, sprains, cuts, bruises, muscle pulls, sore throat, and any pain or inflammation.” • “Use Mode 2 to re-energize brain and heart cells and to normalize brain neuropeptides and heart cell energy.” • “Use Mode 3 as a multi-organ cell re-energizer that cycles through 29 different frequencies proven effective and beneficial for healing, and to benefit inflammation or disorders of all internal, and for the treatment of any unknown condition.” [Note: The proprioceptive points are (1) just in front of the ear over the TMJ, (2) under the angle of the jaw, (3) two inches below the collar bone, and (4) one inch up from the rounded angle of the shoulder blade (scapula).]

www.humanenergyscience.com/uploads/Dr_Lytle_s_audio_4-10.pdf

OSTEOARTHRITIS and the LOW LEVEL LASER
A Transcript of Dr. Lytle’s webinar April 2010

www.21CenturyMedicineChest.com/blog/Joint_Pain
(runtime 59:03) Dr. Irina: Why did you choose osteoarthritis as the basis of your first FDA clinical trials? What were the results of the trials? And we get the exciting news\ www.laserlightinstitute.info/healinglight.pdf

Healing Light by Dr. Larry Lytle
----------------------------------------------
 
21st Century Doc - 21st Century Medicine at your Fingertips

www.scribd.com/doc/66068978/Electrotherapeutic-Devices-Principles-Design-and-Applications

Electrotherapeutic DevicesPrinciples, Design, and Applications --------------------


-------------------------------------------
full pdf

qvchealth.info/clients/931/documents/MuscleRepair_Silveira.pdf
Journal of Photochemistry and Photobiology B: Biology 95 (2009) 89–92

Evaluation of mitochondrial respiratory chain activity in muscle healing by low-level laser therapy

Paulo C.L. Silveira a,*, Luciano Acordi da Silva a, Daiane B. Fraga b, Tiago P. Freitas b, Emilio L. Streck b, Ricardo Pinho a Background: Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process. Objective: In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after traumatic muscular injury. Methods: Male Wistar rats were randomly divided into three groups (n = 6): sham (uninjured muscle), muscle injury without treatment, muscle injury with LLLT (AsGa) 5 J/cm2. Gastrocnemius injury was induced by a single blunt-impact trauma. LLLT was used 2, 12, 24, 48, 72, 96, and 120 hours after muscle- trauma.

Results: Our results showed that the activities of complex II and succinate dehydrogenase after 5 days of muscular lesion were significantly increased when compared to the control group. Moreover, our results showed that LLLT significantly increased the activities of complexes I, II, III, IV and succinate dehydrogenase, when compared to the group of injured muscle without treatment.

Conclusion: These results suggest that the treatment with low-level laser may induce an increase in ATP synthesis, and that this may accelerate the muscle healing process.

www.thorlaser.com/downloads/research/Biphasic-Dose-Response-in-Low-Level-Light-Therapy-Harvard.pdf

Formerly Nonlinearity in Biology, Toxicology, and Medicine Copyright © 2009 University of Massachusetts

BIPHASIC DOSE RESPONSE IN LOW LEVEL LIGHT THERAPY Ying-Ying Huang Michael R. Hamblin, BAR 414, Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston,

www.theragem.com/harvardstudy.pdf www.photobiology.info/Hamblin.html MECHANISMS OF LOW LEVEL LIGHT THERAPY
Michael R. Hamblin Department of Dermatology, Harvard Medical School, BAR 414 Wellman Center for Photomedicine, Massachusetts General Hospital 40 Blossom Street, Boston MA 02114 hamblin@helix.mgh.harvard.edu

www.mgh.harvard.edu/wellman/people/mhamblin.asp
www.photobiology.info/ spie.org/x35504.xml?ArticleID=x35504

Biomedical Optics & Medical Imaging Low-level laser therapy: an emerging clinical paradigm
Ying-Ying Huang, Michael Hamblin, and Aaron C.-H. Chen 9 July 2009, SPIE Newsroom. DOI: 10.1117/2.1200906.1669
Improved understanding of the fundamental cellular and molecular mechanisms is broadening the technique's mainstream use for many ailments. 9 July 2009, SPIE Newsroom. DOI: 10.1117/2.1200906.1669

www.molecularneurodegeneration.com/content/4/1/26 Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy Patricia A Trimmer1*, Kathleen M Schwartz1, M Kathleen Borland1, Luis De Taboada2, Jackson Streeter2 and Uri Oron3

www.photothera.com/technology/neurothera
The NeuroThera® Laser System is under development for the treatment of ischemic stroke and is not approved for sale or distribution in the United States or internationally. The NeuroThera® Laser System is an investigational device that seeks to improve neurological outcomes via noninvasive delivery of near-infrared (NIR) laser energy called Transcranial Laser Therapy (TLT) into the brain. The system consists of a moveable console, a fiber optic cable, and a handpiece. A trained clinician uses the handpiece to direct the TLT to twenty predetermined treatment sites on the patient’s scalp. The total procedure time is approximately 2-3 hours. The Company believes that the NeuroThera® Laser System may offer a compelling option for the treatment of acute ischemic stroke up to twenty-four hours following onset of stroke symptoms.

www.nature.com/jid/journal/v127/n8/full/5700826a.html
www.ncbi.nlm.nih.gov/pubmed/17446900

J Invest Dermatol. 2007 Aug;127(8):2048-57. Epub 2007 Apr 19.

Helium-neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria.

Hu WP, Wang JJ, Yu CL, Lan CC, Chen GS, Yu HS. Source Faculty of Biotechnology and Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Abstract Previous reports have shown that cellular functions could be influenced by visual light (400-700 nm). Recent evidence indicates that cellular proliferation could be triggered by the interaction of a helium-neon laser (He-Ne laser, 632.8 nm) with the mitochondrial photoacceptor-cytochrome c oxidase.

Our previous studies demonstrated that He-Ne irradiation induced an increase in cell proliferation, but not migration, in the melanoma cell line A2058 cell. The aim of this study was to investigate the underlying mechanisms involved in photostimulatory effects induced by an He-Ne laser. Using the A2058 cell as a model for cell proliferation, the photobiologic effects induced by an He-Ne laser were studied. He-Ne irradiation immediately induced an increase in mitochondrial membrane potential (delta psi(mt)), ATP, and cAMP via enhanced cytochrome c oxidase activity and promoted phosphorylation of Jun N-terminal kinase (JNK)/activator protein-1 (AP-1) expressions. He-Ne irradiation-induced A2058 cell proliferation was significantly abrogated by the addition of delta psi(mt) and JNK inhibitors. Moreover, treatment with an He-Ne laser resulted in delayed effects on IL-8 and transforming growth factor-beta1 release from A2058 cells.

These results suggest that He-Ne irradiation elicits photostimulatory effects in mitochondria processes, which involve JNK/AP-1 activation and enhanced growth factor release, and ultimately lead to A2058 cell proliferation.

www.fda.gov/ICECI/EnforcementActions/WarningLetters/ucm246619.htm
March 3, 2011 WARNING LETTER CERTIFIED MAILRETURN RECEIPT REQUESTED
Refer to MIN 11-14 Robert L. Lytle, DDS, PhD President, 2035, Inc.
Managing Limited Partner, QLaser Healing Light LP 520 Kansas City Street, Suite 100 Rapid City, South Dakota 57701

Dear Dr. Lytle: During an inspection of your firm located in Rapid City, South Dakota, on May 25 - 27, 2010, an investigator from the United States Food and Drug Administration (FDA) learned that your firms 2035, Inc. and QLaser Healing Light LP are marketing the Q10 Laser and 808 Enhancer Probe in the United States without marketing clearance or approval, in violation of the Federal Food, Drug, and Cosmetic Act (the Act). Q1000 Laser and 660 Enhancer Probe A review of our records indicates that we cleared a premarket notification (510(k)) for the Q1000 Laser and 660 Enhancer Probe (QLaser System), K080513, with an intended use “for providing temporary relief of pain associated with osteoarthritis of the hand, which has been diagnosed by a physician or other licensed medical professional.”

www.accessdata.fda.gov/cdrh_docs/pdf8/K080513.pdf
Re: K080513 Trade/Device Name: QLaser System Regulation Number: 21 CFR 890.5500 Regulation Name: Infrared lamp Regulatory Class: II Product Code: NHN Dated: January 9, 2009 Received: January 12, 2009

------------------------

lyphedema
- --------------------------

www.riancorp.com/

RianCorp has completed the only randomised double blind clinical trial testing the effects of LLLT on lymphoedema/lymphedema in the world.7 Fleet St, Richmond, South Australia 5033 The LTU-904 models are infra-red lasers operating at a wavelength of 904 nanometers. This invisible wavelength penetrates deeply into tissue (much deeper than the often used red laser operating in the visible red region).

www.rifkinmedicalproducts.com/ RIFKIN MEDICAL PRODUCTS,LLC call 203-758-6569 The LTU-904 laser is only ONLY lymphoedema treatment that has been trialled in a randomised double blind study. The laser works very effectively in about 30% of patients and with some effect in about 50% of patients.


------------------------
www.henrylahore.com/Health/LLLT%20800%20nm%20gets%20into%20spinal%20cord%20July%202009.PDF

 Photomedicine and Laser Surgery Volume 27, Number 3, 2009 ª Mary Ann Liebert, Inc. Pp. 379–380 Guest Editorial*

The Potential of Light Therapy for Central Nervous System Injury and Disease Juanita J. Anders, Ph.D. Department of Anatomy, Physiology and Genetics Uniformed Services University of the Health Sciences 4301 Jones Bridge Rd. Bethesda, MD 20814 Juanita J. Anders

--------------------------------

www.isan.troitsk.ru/dls/publ/tiinaabs2005.pdf
 
Absorption measurements of a cell monolayer relevant to phototherapy: Reduction of cytochrome c oxidase under near IR radiation Tiina I. Karu a,*, Lydmila V. Pyatibrat a, Sergei F. Kolyakov b, Natalya I. Afanasyeva c
Journal of Photochemistry and Photobiology B: Biology 81 (2005) 98–106 a Institute of Laser and Information Technologies of Russian Academy of Sciences, Troitsk, Pionerskaya Street 2, Moscow Region 142190, Russian Federation b Institute of Spectroscopy of Russian Academy of Sciences, Troitsk, Moscow Region 142190, Russian Federation c Spectrooptical Sensing Inc., Portland, OR 97205, USA Received 26 April 2005; accepted 20 July 2005

Phototherapy uses monochromatic light in the optical region of 600–1000 nm to treat in a non-destructive and non-thermal fashion various soft-tissue and neurological conditions. This kind of treatment is based on the ability of light red-to-near IR to alter cellular metabolism as a result of its being absorbed by cytochrome c oxidase. To further investigate the involvement of cytochrome c oxidase as a photoacceptor in the alteration of the cellular metabolism,


Jeffrey Dach MD
7450 Griffin Road, SUite 190
Davie, Fl 33314
954-792-4663
www.jeffreydach.com
www.drdach.com
www.naturalmedicine101.com
www.bioidenticalhormones101.com
www.truemedmd.com

Click Here for: Dr Dach's Online Store for Pure Encapsulations Supplements
Click Here for: Dr Dach's Online Store for Nature's Sunshine Supplements

Web Site and Discussion Board Links:

jdach1.typepad.com/blog/ disc.yourwebapps.com/Indices/244124.html
disc.yourwebapps.com/Indices/244066.html
disc.yourwebapps.com/Indices/244067.html
disc.yourwebapps.com/Indices/244161.html
disc.yourwebapps.com/Indices/244163.html


Disclaimer click here: www.drdach.com/wst_page20.html

The reader is advised to discuss the comments on these pages with his/her personal physicians and to only act upon the advice of his/her personal physician. Also note that concerning an answer which appears as an electronically posted question, I am NOT creating a physician -- patient relationship. Although identities will remain confidential as much as possible, as I can not control the media, I can not take responsibility for any breaches of confidentiality that may occur.

Link to this article:

http://jeffreydach.com/2011/12/02/low-level-laser-medicine-of-the-future.aspx

Copyright (c) 2011 Jeffrey Dach MD All Rights Reserved. This article may be reproduced on the internet without permission, provided there is a link to this page and proper credit is given.

FAIR USE NOTICE: This site contains copyrighted material the use of which has not always been specifically authorized by the copyright owner. We are making such material available in our efforts to advance understanding of issues of significance. We believe this constitutes a 'fair use' of any such copyrighted material as provided for in section 107 of the US Copyright Law. In accordance with Title 17 U.S.C. Section 107, the material on this site is distributed without profit to those who have expressed a prior interest in receiving the included information for research and educational purposes.





(c)Copyright 2011 All Rights Reserved Jeffrey Dach MD, Bioidentical Hormone Blog 7450 Griffin Road, Suite 190, Davie, Florida 33314
Post a comment
Write a comment:

Related Searches