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X Ray Microscope

Posted Dec 28 2010 11:17pm


An X-ray microscope is used for producing images of “smaller objects”. X-ray posses the property of not reflecting and therefore cannot be seen by the human eyes. The use of an X-ray microscope is to detect these invisible X-rays that “pass through the specimen” with the help of an “expose film” or with the use of a “charged-coupled device” known as the “CCD”. This technology is based on the concept of “contrast imaging” that is obtained through the “absorption of soft X-rays”.

In the early days, X-ray microscopes were based on “grazing-incidence reflective optics” which was used for focusing of the “X-rays off parabolic curved mirrors”, which possessed very “high angle of incidence”. a “tiny fresnel zonal plate” which characterized of “concentric rings of gold and silver” can also be used on a “silicon dioxide” to focus the X-ray and is the alternative method which is used for this purpose.

In 1950’s “shadow X-ray microscopes” was first to be termed as “commercial X-ray microscopes” presented by the “General Electric Company”. This X-ray microscope was used by placing the specimen “between the source and the target plate”.

The X-ray microscope which is presently use known as the “Berkeley’s XM-1″ is based on the “X-ray lens” that focuses the X-rays on a “CCD”, which is very similar to the functioning of an “optical microscope”. The first X-ray microscope that was used for “biological as well as the biomedical research”, featuring soft X-ray microscope was designed by “ALS”.

The source like the “synchrotron radiation sources” of this soft X-ray, “suitable for microscopy” had comparatively low “brightness of the required wavelengths”. Therefore, an alternative method for the image formation was developed, which involves the use of “scanning transmission soft X-ray microscopy”, which is based on the concept of focusing the X-rays on a point. This sample is then “mechanically scanned” with the help of focal point which is produced. This focal point records all the transmitted X-ray at “each point” with the help of a detector like the “proportional counter” or the “avalanche photodiode”. The “Stony Brook University” researchers developed this method, which was used at the “National Synchronoton Light Source” situated at the “Brookhaven national Laboratory”.

The X-ray microscopes were useful as compared to the “conventional electron microscopy” for better viewing of the “biological samples in their natural state”, which were largely used to obtain images with the “nanometer level resolution”.

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