It is possible to quantitate precisely the level of plasma insulin by the epochal technique of radioimmunoassay, devised by Yalow and Berson for which Dr. Rosalyn Yalow received the Nobel Prize in 1977, Dr. Solomon Berson regrettably having died. A similar immunoassay is available to determine levels of circulating peptides, quantitation of which provides a means of documenting beta cell secretory function in diabetic patients who must perforce receive exogenous insulin therapy. Insulin release in response to some stimulus is a biphasic phenomenon, reflecting probably a two-pool. system within the beta cell. A prompt or immediate release probably involves insulin that is stored within secretory granules. A more delayed, chronically released pool could be derived from stored proinsulin, requiring conversion to insulin, as well as from activation of the ribosomal system, with synthesis of more insulin. medical billing training india. will help you to do collections and also the pending amounts from the patients.