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VAC-BAC Shuttle Vector System for Generating Recombinant Poxviruses

Posted Dec 27 2009 4:00pm

Description of Invention:
This invention relates to a VAC-BAC shuttle vector system for the creation of recombinant poxviruses from DNA cloned in a bacterial artificial chromosome. A VAC-BAC is a bacterial artificial chromosome (BAC) containing a vaccinia virus genome (VAC) that can replicate in bacteria and produce infectious virus in mammalian cells.

Applications:
  • VAC-BACs can be used to modify vaccinia virus DNA by deletion, insertion or point mutation or add new DNA to the VAC genome with methods developed for bacterial plasmids, rather than by recombination in mammalian cells.
  • It can be used to produce recombinant vaccinia viruses for gene expression.
  • It can be used for the production of modified vaccinia viruses that have improved safety or immunogenicity.


Advantages:
  • VAC-BACs are clonally purified from bacterial colonies before virus reconstitution in mammalian cells.
  • Manipulation of DNA is much simpler and faster in bacteria than in mammalian cells.
  • Modified genomes can be characterized prior to virus reconstitution.
  • Only virus with modified genomes will be produced so that virus plaque isolations are not needed.
  • Generation of a stock of virus from a VAC-BAC is accomplished within a week rather than many weeks.
  • Multiple viruses can be generated at the same time since plaque purification is unnecessary.


Inventors:
Bernard Moss (NIAID)
Arban Domi (NIAID)


Patent Status:
HHS, Reference No. E-355-2001/2
US, , Patent No. 7,494,813, Issued 24 Feb 2009
PCT, Application No. PCT/US03/11183 filed 10 Apr 2003


Relevant Publication:
  1. A Domi and B Moss. Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells. Proc Natl Acad Sci USA. 2002 Sep 17;99(19):12415-12420. [ PubMed: 12196634 ]
  2. A Domi and B Moss. Engineering of a vaccinia virus bacterial artificial chromosome in Escherichia coli by bacteriophage lambda-based recombination. Nat Methods. 2005 Feb;2(2):95-97. [ PubMed: 15782205 ]


Licensing Status:
Available for licensing.


Portfolios:
Infectious Diseases
Infectious Diseases - Therapeutics
Infectious Diseases - Vaccines



For Additional Information Please Contact:
Susan Ano Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: anos@mail.nih.gov
Phone: 301-435-5515
Fax: 301-402-0220


Ref No: 672

Updated: 12/2009

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