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Simultaneous Detection of Non-pneumophila Legionella Strains Using Real-time PCR

Posted Aug 01 2013 8:00pm

Description of Invention:
Legionnaires' disease is caused by a type of bacteria called Legionella. CDC scientists have developed a real-time multiplex PCR assay for diagnosis and identification of Legionella strains. The assay consists of five sets of primers (targeting L. bozemanii, L. dumoffii, L. feeleii, L. longbeachae, or L. micdadei) and corresponding probes. Each probe is labeled with a different fluorophore which allows the detection of a particular strain in a single tube reaction. Using this assay format, the presence of any one of the five pathogenic non-pneumophila strains of Legionella can be detected rapidly from clinical or environmental samples. Rapid and sensitive identification enables initiation of appropriate antibiotic therapy and identification of the source of bacteria so that proper public health responses may occur.

Applications:
  • Rapid and real-time assay to detect the presence of clinically relevant non-pneumophila Legionella strains.


Advantages:
  • Currently available tests are time consuming and labor intensive.
  • This assay enables rapid identification and differentiation on clinically relevant non-pneumophila Legionella strains.
  • This assay can be used as a standalone confirmatory assay for the detection of common non-pneumophila Legionella species or as one of the valuable assays in conjunction with other standard assays.


Development Status:
  • Pre-clinical
  • In vitro data available


Inventors:
Jonas M Winchell (CDC)
Alvaro J Benitez (CDC)


Patent Status:
HHS, Reference No. E-277-2013/0
PCT, Application No. PCT/US2013/030217 filed 11 Mar 2013
US, Application No. 13/895,898 filed 16 May 2013


Relevant Publication:
  1. Benitez AJ, Winchell JM. [ PMID 23135949 ]



For Licensing Information Please Contact:
Whitney Blair
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: blairw2@mail.nih.gov
Phone: 301-435-5031
Fax: 301-402-0220


Ref No: 2599

Updated: 08/2013

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