Simultaneous Detection of Non-pneumophila Legionella Strains Using Real-time PCR
Posted Aug 01 2013 8:00pm
Description of Invention: Legionnaires' disease is caused by a type of bacteria called Legionella. CDC scientists have developed a real-time multiplex PCR assay for diagnosis and identification of Legionella strains. The assay consists of five sets of primers (targeting L. bozemanii, L. dumoffii, L. feeleii, L. longbeachae, or L. micdadei) and corresponding probes. Each probe is labeled with a different fluorophore which allows the detection of a particular strain in a single tube reaction. Using this assay format, the presence of any one of the five pathogenic non-pneumophila strains of Legionella can be detected rapidly from clinical or environmental samples. Rapid and sensitive identification enables initiation of appropriate antibiotic therapy and identification of the source of bacteria so that proper public health responses may occur.
Rapid and real-time assay to detect the presence of clinically relevant non-pneumophila Legionella strains.
Currently available tests are time consuming and labor intensive.
This assay enables rapid identification and differentiation on clinically relevant non-pneumophila Legionella strains.
This assay can be used as a standalone confirmatory assay for the detection of common non-pneumophila Legionella species or as one of the valuable assays in conjunction with other standard assays.
In vitro data available
Inventors: Jonas M Winchell (CDC) Alvaro J Benitez (CDC)
For Licensing Information Please Contact: Whitney Blair NIH Office of Technology Transfer 6011 Executive Blvd. Suite 325, Rockville, MD 20852 United States Email: firstname.lastname@example.org Phone: 301-435-5031 Fax: 301-402-0220