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Retinitis Pigmentosa Update: Three Blind Mice – Let There Be Sight!

Posted Apr 19 2012 8:09pm
In a potential breakthrough, especially for those suffering from retinitis pigmentosa who have lost vision due to degenerated photoreceptors in their retina, scientists from the University College of London (UCL) Institute of Ophthalmology have managed to transfer immature (or progenitor) rod-photoreceptor cells – cells that are more developed than stem cells, but not quite mature rod cells – from healthy mice into those suffering from deficiencies in photoreceptors (blind mice) and, after a few weeks, have found that the transplanted cells appeared to be functioning almost as well as normal rod-photoreceptor cells and had formed the connections needed to transmit visual information to the brain.

This is very early work along a long and tedious path to, hopefully, eventual success, but a small step in the right direction. The ultimate goal would be to use embryonic stem cells to grow both rod and cone cells for transplantation into humans suffering from degenerated cone and rod cells.

This potential breakthrough could mean hope for thousands of people with photoreceptor deficiencies (and blindness), because of diseases such as age-related macular degeneration, retinitis pigmentosa and diabetes-related blindness.
The results of the study have just been published in Nature (April 18), “Restoration of Vision After Transplantation of Photoreceptors” .
More information about this discovery can be read in the news release put out by UCL:

Photoreceptor transplant restores vision in mice18 April 2012
Scientists from the UCL Institute of Ophthalmology have shown for the first time that transplanting light-sensitive photoreceptors into the eyes of visually impaired mice can restore their vision. The research, published in Nature, suggests that transplanting photoreceptors - light-sensitive nerve cells that line the back of the eye - could form the basis of a new treatment to restore sight in people with degenerative eye diseases.
Scientists injected cells from young healthy mice directly into the retinas of adult mice that lacked functional rod-photoreceptors. Loss of photoreceptors is the cause of blindness in many human eye diseases including age-related macular degeneration, retinitis pigmentosa and diabetes-related blindness.
There are two types of photoreceptor in the eye - rods and cones. The cells transplanted were immature (or progenitor) rod-photoreceptor cells. Rod cells are especially important for seeing in the dark as they are extremely sensitive to even low levels of light.
After four to six weeks, the transplanted cells appeared to be functioning almost as well as normal rod-photoreceptor cells and had formed the connections needed to transmit visual information to the brain.
Transplanted Photoreceptors

The researchers also tested the vision of the treated mice in a dimly lit maze. Those mice with newly transplanted rod cells were able to use a visual cue to quickly find a hidden platform in the maze whereas untreated mice were able to find the hidden platform only by chance after extensive exploration of the maze.
Professor Robin Ali at UCL Institute of Ophthalmology, who led the research, said: "We've shown for the first time that transplanted photoreceptor cells can integrate successfully with the existing retinal circuitry and truly improve vision. We're hopeful that we will soon be able to replicate this success with photoreceptors derived from embryonic stem cells and eventually to develop human trials."
"Although there are many more steps before this approach will be available to patients, it could lead to treatments for thousands of people who have lost their sight through degenerative eye disorders. The findings also pave the way for techniques to repair the central nervous system as they demonstrate the brain's amazing ability to connect with newly transplanted neurons."
Dr Rachael Pearson from UCL Institute of Ophthalmology and principal author, said: "We are now finding ways to improve the efficiency of cone photoreceptor transplantation and to increase the effectiveness of transplantation in very degenerate retina. We will probably need to do both in order to develop effective treatments for patients."
Dr Rob Buckle, head of regenerative medicine at the Medical Research Council (MRC) said: "This is a landmark study that will inform future research across a wide range of fields including vision research, neuroscience and regenerative medicine. It provides clear evidence of functional recovery in the damaged eye through cell transplantation, providing great encouragement for the development of stem cell therapies to address the many debilitating eye conditions that affect millions worldwide."
The researchers demonstrated previously, in another study published in Nature,  that it is possible to transplant photoreceptor cells into an adult mouse retina, provided the cells from the donor mouse are at a specific stage of development - when the retina is almost, but not fully, formed. In this study they optimised the rod transplantation procedure to increase the number of cells integrated into the recipient mice and so were able to restore vision.
The research was funded by the MRC, the Wellcome Trust, the Royal Society, the British Retinitis Pigmentosa Society, Alcon Research Institute and The Miller's Trust. Robin Ali is a senior investigator of the National Institute for Health Research and carries out research at the NIHR Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology. Rachael Pearson is a Royal Society University Research Fellow.

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