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Real-time TaqMan RT-PCR Assays for Selective Detection of Human Rhinovirus

Posted Aug 12 2013 8:00pm

Description of Invention:
This invention relates to selective detection of human rhinovirus (HRV) in biological media. Specifically, this invention discloses a real-time TaqMan RT-PCR assay targeting the 5'-noncoding region of the HRV genome. This is a one-step, real-time nucleic acid assay that offers rapid, sensitive, and quantitative results. The assay is validated against all 100 recognized HRV prototype strains.

HRV is the most frequent cause of the common cold. From a clinical standpoint, diagnosis of HRV infection is quite difficult as the related symptoms can be caused by other agents as well. Additionally, laboratory detection of HRV is challenging as HRV exhibits extreme antigenic variability and certain strains cannot be maintained by cell culture.

Applications:
  • Development of human rhinovirus (HRV) diagnostics
  • Acute lower respiratory illness diagnostics and investigation


Advantages:
  • Validated against all 100 human rhinovirus prototype strains
  • Rapid, sensitive and quantitative
  • One-step assay
  • Easily adapted to kit form


Development Status:
  • Early-stage
  • In vitro data available


  • Inventors:
    Xiaoyan Lu (CDC)
    Dean D Erdman (CDC)


    Patent Status:
    HHS, Reference No. E-177-2013/0
    US, Application No. 12/315,758 filed 05 Dec 2008


    Relevant Publication:
    1. Lu X, et al. [ PMID 18057136 ]



    For Licensing Information Please Contact:
    Whitney Blair
    NIH Office of Technology Transfer
    6011 Executive Blvd. Suite 325,
    Rockville, MD 20852
    United States
    Email: blairw2@mail.nih.gov
    Phone: 301-435-4937
    Fax: 301-402-0220


    Ref No: 2612

    Updated: 08/2013

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