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Rapid Detection of Multi-Drug-Resistant Mycobacterium Tuberculosis Using Real-Time PCR and High-Resolution Melt Analysis

Posted Aug 12 2013 8:00pm

Description of Invention:
CDC scientists have developed a rapid, sensitive, and specific real-time PCR assay that is capable of detecting the presence of Mycobacterium tuberculosis and determining its resistance profile to antibiotics, such as rifampicin and isoniazid. Currently, there are few assays available that are capable of both detecting M. tuberculosis and determining the bacteria's drug resistance. This assay incorporates multiple fluorescent chemistries, providing a simple and cost-effective method of determining the bacteria's drug resistance. Additionally, this assay may be used to quickly discriminate Mycobacterium tuberculosis complex (MTBC) strains from non-MTBC strains.

  • Rapid screening of potential multi-drug-resistant M. tuberculosis
  • Kits for diagnosis of M. tuberculosis
  • Public health programs combating emerging drug-resistance in M. tuberculosis; clinics working with at-risk populations

  • Robust and inexpensive way to detect dominant M. tuberculosis mutations
  • Rapid results within 5 hours of obtaining DNA
  • More cost-efficient and less complex than culturing and sequencing methods of determining drug-resistant status

Development Status:
  • Early-stage
  • In vitro data available

James E Posey (CDC)
Jonas M Winchell (CDC)
Kelley Cowart (CDC)
Melissia Ramirez (CDC)

Patent Status:
HHS, Reference No. E-160-2013/0
US, Application No. 13/695,935 filed 02 Nov 2012
PCT, Application No. PCT/US2011/035217 filed 04 May 2011

Relevant Publication:
  1. Ramirez MV, et al. [ PMID 20810777 ]

For Licensing Information Please Contact:
Whitney Blair
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Phone: 301-435-4937
Fax: 301-402-0220

Ref No: 2611

Updated: 08/2013

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