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Polyclonal Antibodies against RGS7, a Regulator of G Protein Signaling, for Research and Diagnostic Use

Posted Jun 14 2011 8:00pm

Description of Invention:
Investigators at the National Institutes of Health have generated a polyclonal antibody against the Regulator of G protein Signaling Protein 7 (RGS7). The RGS7 protein regulates neuronal G protein signaling pathways and inhibits signal transduction by increasing the GTPase activity of G protein alpha. RGS7 may play an important role in synaptic vesicle exocytosis and in the rapid regulation of neuronal excitability and the cellular responses to stimulation. This polyclonal antibody was generated by using a purified fusion protein containing the regulator of guanine nucleotide-binding protein signaling (RGS) C-terminal region of bovine RGS. The antibody specifically recognizes RGS7 of mouse, rat, and human origin. The antibody is useful for studying the expression, functions, and interactions of RGS7 by Western blot and immunofluorescence analysis.

Applications:
  • Basic research tool for the study of RGS7. Reagent for diagnostic applications such as Western Blotting, ELISA, immunofluorescence and immunohistochemistry in fixed tissue samples.
  • Reagent for biochemical techniques such as immunoprecipitation. Development of diagnostics or therapeutics for diseases of the nervous system linked to RGS protein-regulated signaling including Parkinson’s disease, schizophrenia, seizure disorders, multiple sclerosis, and opiate addiction.


Inventors:
William F Simonds (NIDDK)
Jianhua Zhang (NIDDK)


Patent Status:
HHS, Reference No. E-077-2011/0

Research Tool — Patent protection is not being pursued for this technology.

Relevant Publication:
  1. Rojkova AM, Woodard GE, Huang TC, Combs CA, Zhang JH, Simonds WF. Ggamma subunit-selective G protein beta 5 mutant defines regulators of G protein signaling protein binding requirement for nuclear localization. J Biol Chem. 2003 Apr 4;278(14):12507-12512. [ PMID: 12551930 ]
  2. Nini L, Waheed AA, Panicker LM, Czapiga M, Zhang JH, Simonds WF. R7-binding protein targets the G protein beta 5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain. BMC Biochem. 2007 Sep 19;8:18. [ PMID: 17880698 ]
  3. Panicker LM, Zhang JH, Posokhova E, Gastinger MJ, Martemyanov KA, Simonds WF. Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein. J Neurochem. 2010 Jun;113(5):1101-1112. [ PMID: 20100282 ]


Licensing Status:
This technology is available as a research tool under a Biological Materials License.

Collaborative Research Opportunity:
The NIDDK Metabolic Diseases Branch is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize polyclonal antibodies against the Regulator of G protein Signaling Protein 7 (RGS7). Please contact Anna Z. Amar at 301-451-2305 or aa54d@nih.gov for more information.


Portfolios:
Research Materials



For Licensing Information Please Contact:
Jaime Greene M.S.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: greenejaime@mail.nih.gov
Phone: 301-435-5559
Fax: 301-402-0220


Ref No: 2266

Updated: 06/2011

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