p-Toluemesulfonhydrazide Derivatization for Separation and Measurement of Endogenous Estrogen Metabolites by High-Pressure Liqui

Description of Invention:
The current invention relates to a method for measuring endogenous estrogen levels, and this technology may be generalizable to all endogenous ketolic steroids, including estrogens, androgens, and phytoestrogens.

Specifically, the current invention is a derivatization technique that forms estrogen-p-toluenesulfonhydrazones, which can be separated and then measured using high-pressure liquid chromatography-electrospray-mass spectrometry (HPLC-ESI-MS). This method offers a number of improvements over current methods. It is more sensitive, it is faster, it is more accurate, and it requires a smaller sample size.

Inventors:
Regina G Ziegler (NCI)
David J Waterhouse (NCI)
Joseph E Saavedra (NCI)
Larry K Keefer (NCI)
Xia Xu (NCI)


Patent Status:
HHS, Reference No. E-103-2002/0
US, Application No. 10/511,409 filed 13 Oct 2004



Portfolios:
Internal Medicine
Internal Medicine - Research Materials



For Additional Information Please Contact:
Michael Shmilovich Esq.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: shmilovm@mail.nih.gov
Phone: 301-435-5019
Fax: 301-402-0220


Ref No: 688

Updated: 05/2004