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Method and Platform for Selectively Labeling RNA

Posted Aug 21 2013 8:00pm

Description of Invention:
The invention pertains to a three step initiation, elongation and termination method and platform for synthesizing selectively labeled RNA molecules by first polymerizing a first liquid phase RNA molecule from a solid phased DNA template fixed onto a solid phase. The method includes the steps of incubating the solid and liquid phases at appropriate elongation temperatures and then terminating elongation by a separation stage where the phases are incubated at near 0 degrees Celsius where it selectively terminates RNA elongation. The steps can be repeated by the number bases (rNTPs) in the final RNA molecule wherein in each iterative stage a new rNTP can be added that is selectively labeled. The DNA may have a density of 30-80% on the solid substrate, and the solid substrate may be a bead. The bead may comprise a gel, glass, or a synthetic polymer. The bead may have a diameter of 5-100 mm. The concentration of DNA may be 30 mm-1 nm. The concentration of rNTP may be 1-100 times the DNA concentration. The RNA polymerase may be a T7 RNA polymerase. The label may be 13C/5N, 2H, Cy3, Cy5, a fluorophore, a heavy atom, or a chemical modification.

Differentially labeled diagnostics

Multiple use detection method

Development Status:
  • Prototype
  • In vitro data available

Yun-Xing Wang (NCI)
Liu Yu (NCI)
Ping Yu (NCI)
Rui Sousa (University of Texas Health Science Center)

Patent Status:
HHS, Reference No. E-119-2013/0
US, Application No. 61/843,864 filed 08 Jul 2013

Relevant Publication:
  1. Guajardo R, Sousa R. [ PMID 8995520 ]
  2. Guo Q, et al. [ PMID 16169559 ]
  3. Mukherjee S, et al. [ PMID 12150999 ]
  4. Mentesana PE, et al. [ PMID 11183774 ]

For Licensing Information Please Contact:
Michael Shmilovich Esq.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Phone: 301-435-5019
Fax: 301-402-0220

Ref No: 2618

Updated: 08/2013

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