Live Attenuated Rubella Vector to Express Vaccine Antigens
Posted Feb 04 2013 7:00pm
Description of Invention: Live attenuated viruses make potent and effective vaccines. Despite the urgent need for an HIV vaccine, this approach has not been feasible because it has not been possible to attenuate the virus reliably and guarantee vaccine safety. Instead, live viral vectors have been proposed that could present HIV vaccine antigens in the most immunogenic way, in the context of an active infection.
The inventors have adapted a rubella vaccine strain as a vector to express HIV and SIV antigen and tested the effect of insert size and composition on vector stability and viral titer. The inventors have identified an acceptor site in the rubella nonstructural gene region, where foreign genes can be expressed as a fusion protein with the nonstructural protein P150 without affecting essential viral functions. The inserts were expressed as early genes of rubella, under control of the rubella genomic promoter. At this site, HIV and SIV antigens were expressed stably for at least seven passages, as the rubella vectors reached high titers. Rubella readily infects rhesus macaques, and these animals will provide an ideal model for testing the new vectors for replication in vivo, immunogenicity and protection against SIV or SHIV challenge.
Ease of manufacture
Low cost vaccines
In vitro data available
In vivo data available (animal)
Inventors: Ira Berkower (FDA) Konstantin Virnik (FDA)
For Licensing Information Please Contact: Peter Soukas J.D. NIH Office of Technology Transfer 6011 Executive Blvd. Suite 325
Room 14, Rockville, MD 20852-3804 United States Email: email@example.com Phone: 301-435-4646 Fax: 301-402-0220