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Lentiviral Vectors with Dual Fluorescence/Luminescence Reporters

Posted Apr 07 2013 8:00pm

Description of Invention:
Twelve lentiviral vectors that express both fluorescent and luminescent markers as a single fusion protein under various gene promoters were constructed. Vectors have been developed previously to monitor tumors or tumor cells via bioluminescence or fluorescence alone. However, bioluminescence is not sensitive enough to sort individual tumor cells and fluorescence cannot be used effectively to view internal tumors. By combining the two reporters into a single fusion protein, the tumor can be effectively visualized within the animal as well as sorted from non-tumor cells for post-necropsy experiments. The added advantage of bioluminescent visualization allows for in vivo experiments that more closely simulate the biological development of tumors in organs rather than at the surface of the skin. Additionally, since twelve different vectors with different gene promoters were developed, they can be tested in individual tumor models to find the best vector for visualizing that particular tumor cell line. The vectors are able to sustain long-term expression of both visualization markers, depending on the cell type and promoter in each vector.

Applications:
  • The vectors will be extremely useful for experiments in which both in vivo and in vitro analysis is desired.
  • The vectors can also be used for screening cancer cell lines and in tumor models for reporter gene activity.
  • The vectors can be useful in drug development.


Advantages:
  • The bioluminescent marker allows for effective visualization of deep (non-surface) tumors in mice.
  • The fluorescence label permits efficient sorting of tumor cells from normal (non-labeled) cells after tumors are excised from the mice.
  • The vectors allow in vivo experiments that more closely simulate the biological development of tumors in organs rather than at surface of skin.
  • The vectors sustain long-term expression.


Development Status:
  • Early-stage
  • Pre-clinical
  • In vitro data available
  • In vivo data available (animal)


Inventors:
Dominic Esposito (NCI)
Chi-Ping Day (NCI)
Glenn T Merlino (NCI)


Patent Status:
HHS, Reference No. E-132-2011/0

Research Tool – Patent protection is not being pursued for this technology.

Relevant Publication:
  1. Day CP, et al. [ PMID 19175523 ]


Collaborative Research Opportunity:
The National Cancer Institute is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize dual luminescent/fluorescent vectors. For collaboration opportunities, please contact John D. Hewes, Ph.D. at hewesj@mail.nih.gov .


For Licensing Information Please Contact:
Suryanarayana Vepa Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: vepas@mail.nih.gov
Phone: 301-435-5020
Fax: 301-402-0220


Ref No: 2550

Updated: 04/2013

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