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Increased Therapeutic Effectiveness of Immunotoxins That Use Toxin Domains Lacking Both T-cell and B-cell Epitopes

Posted Feb 07 2012 7:00pm

Description of Invention:
Immunotoxins can kill cancer cells while allowing healthy, essential cells to survive. As a result, patients receiving an immunotoxin are less likely to experience the deleterious side-effects associated with non-discriminate therapies such as chemotherapy or radiation therapy. Unfortunately, the continued administration of immunotoxins often leads to a reduced patient response due to the formation of neutralizing antibodies against immunogenic B-cell and T-cell epitopes contained within PE. To improve the therapeutic effectiveness of PE-containing immunotoxins through multiple rounds of drug administration, NIH inventors have sought to remove the B-cell and T-cell epitopes within PE. Previous work demonstrated that the removal of the major B-cell epitopes from PE reduced the immunogenicity of PE. This technology involves the identification of major T-cell epitopes on PE, and the removal of the primary T-cell epitope by mutation or deletion. By combining the T-cell epitope mutations with modifications that remove B-cell epitopes, it is possible to create PE-based immunotoxins that have even greater resistance to the formation of neutralizing antibodies. Immunotoxins containing these new PE-variants are expected to have improved therapeutic efficacy.

Applications:
  • Essential component of immunotoxins
  • Treatment of any disease associated with increased or preferential expression a specific cell surface receptor
  • Specific diseases include hematological cancers, lung cancer, ovarian cancer, breast cancer, and head and neck cancers


Advantages:
  • PE variants now include the removal of both B-cell and T-cell epitopes, further reducing the formation of neutralizing antibodies against immunotoxins which contain the PE variants
  • Less immunogenic immunotoxins result in improved therapeutic efficacy by permitting multiple rounds of administration
  • Targeted therapy decreases non-specific killing of healthy, essential cells, resulting in fewer non-specific side-effects and healthier patients


Development Status:
Pre-clinical

Inventors:
Ira H Pastan (NCI)


Patent Status:
HHS, Reference No. E-174-2011/0
US, Application No. 61/495,085 filed 09 Jun 2011


Related Technologies:
PCT, Application No. PCT/US2010/048504 filed 10 Sep 2010, Reference No. E-269-2009/0
US, Application No. 12/676,203 filed 04 Sep 2008, Reference No. E-292-2007/0
US, Application No. 11/997,202 filed 25 Jul 2006, Reference No. E-262-2005/0
US, Patent No. 7,081,518, Issued 25 Jul 2006, Reference No. E-139-1999/0
US, Application No. 60/160,071 filed 27 May 1999, Reference No. E-139-1999/0
US, Application No. 60/703,798 filed 29 Jul 2005, Reference No. E-262-2005/0
US, Application No. 60/969,929 filed 04 Sep 2007, Reference No. E-292-2007/0
US, Application No. 61/241,620 filed 11 Sep 2009, Reference No. E-269-2009/0


and multiple additional patent families


For Licensing Information Please Contact:
David Lambertson Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: lambertsond@mail.nih.gov
Phone: 301-435-4632
Fax: 301-402-0220


Ref No: 2361

Updated: 02/2012

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