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High-level Expression and Purification of Untagged and Histidine-tagged Human Immunodeficiency Virus type-1 (HIV-1) Reverse Tran

Posted Jun 13 2010 5:00pm

Description of Invention:
This invention includes plasmids and protocols to express and purify large quantities of histidine-tagged and untagged HIV-1 reverse transcriptase (RT). Conditions have been optimized for overexpression and purification of p66 and p51 heterodimer RT in E. coli. High-level of expression was reached as RT represented approximately 30%-40% of total cell proteins. The subject invention enables the purification of large quantities of heterodimer RT necessary for structural and kinetic studies and facilitates subunit-specific amino acid alterations essential for structure/function investigations.

Applications:
Research Tool

Development Status:
In vitro data available

Inventors:
Samuel H Wilson (NIEHS)
Rajendra Prasad (NIEHS)
Ester W Hou (NIEHS)


Patent Status:
Research Tool -- patent protection is not being pursued for this technology

Relevant Publication:
  1. EW Hou, R Prasad, WA Beard, SH Wilson. High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase. Protein Expr Purif. 2004 Mar;34(1):75-86. [ PubMed abs ]


Licensing Status:
Available for non-exclusive licensing as biological material and research tool.


Portfolios:
Infectious Diseases
Infectious Diseases - Research Materials
Infectious Diseases - Other
In-vitro Data



For Additional Information Please Contact:
Sally Hu Ph.D., M.B.A.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325 Room 21,
Rockville, MD 20852
United States
Email: hus@mail.nih.gov
Phone: 301-435-5606
Fax: 301-402-0220


Ref No: 1517

Updated: 06/2010

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