Description of Invention: Calcium is a key element in the regulation of many cellular processes, including muscle contraction, hormone excretion from gland cells, neurotransmitter release from nerve synapses, and the regulation of cellular metabolism. Elevated calcium levels are found in a number of diseases.
The present invention relates to chromophoric or fluorescent dye calcium indicators that are superior for measurement of high concentrations of calcium ions due to their high dissociation constants. As a result of the high calcium ion dissociation constants, the perturbation resulting from introducing the indicator into the cell is greatly reduced. These calcium ion indicators can be measured by various techniques including 19F NMR spectroscopy, flow cytometry, and quantitative fluorescence techniques, and are useful for measuring calcium levels within the cytosol or within cellular organelles.
Applications: Research tool for quantifying intracellular calcium concentrations.
Inventors: Robert E London (NIEHS) Louis A Levy (NIEHS) Elizabeth Murphy (NIEHS)
Licensing Status: Available for nonexclusive licensing.
Collaborative Research Opportunity: The NIEHS Laboratory of Structural Biology is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize this technology. Please contact Dr. Robert London at 919/541-4879 or london@niehs.nih.gov for more information.
Portfolios: Internal Medicine Internal Medicine - Diagnostics
For Additional Information Please Contact: Suryanarayana Vepa Ph.D. NIH Office of Technology Transfer 6011 Executive Blvd. Suite 325, Rockville, MD 20852 United States Email: vepas@mail.nih.gov Phone: 301-435-5020 Fax: 301-402-0220
Description of Invention:
Calcium is a key element in the regulation of many cellular processes, including muscle contraction, hormone excretion from gland cells, neurotransmitter release from nerve synapses, and the regulation of cellular metabolism. Elevated calcium levels are found in a number of diseases.
The present invention relates to chromophoric or fluorescent dye calcium indicators that are superior for measurement of high concentrations of calcium ions due to their high dissociation constants. As a result of the high calcium ion dissociation constants, the perturbation resulting from introducing the indicator into the cell is greatly reduced. These calcium ion indicators can be measured by various techniques including 19F NMR spectroscopy, flow cytometry, and quantitative fluorescence techniques, and are useful for measuring calcium levels within the cytosol or within cellular organelles.
Applications:
Research tool for quantifying intracellular calcium concentrations.
Inventors:
Robert E London (NIEHS)
Louis A Levy (NIEHS)
Elizabeth Murphy (NIEHS)
Patent Status:
HHS, Reference No. E-015-1993/0
US, , Patent No. 5,516,911, Issued 14 May 1996
Licensing Status:
Available for nonexclusive licensing.
Collaborative Research Opportunity:
The NIEHS Laboratory of Structural Biology is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize this technology. Please contact Dr. Robert London at 919/541-4879 or london@niehs.nih.gov for more information.
Portfolios:
Internal Medicine
Internal Medicine - Diagnostics
For Additional Information Please Contact:
Suryanarayana Vepa Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: vepas@mail.nih.gov
Phone: 301-435-5020
Fax: 301-402-0220
Ref No: 1515
Updated: 03/2007