Description of Invention: Available for licensing and commercial development are methods, articles, software and kits for determining the spectrum of peptidyl sequences that are recognized and phosphorylated by a kinase, such as those sites on proteins involved in signal transduction pathways. More specifically, the following is disclosed:
methods involving a degenerate library approaches to identify kinase specificity by identifying peptide sequences around such phosphorylation sites and ranking the peptides in preferential order after calculating a predictive score, such as the widely used position-specific scoring matrix (PSSM). The method also provides an informative graphical format for visually representing that information and software to output data in that format. The method provides significant improvements over other methods currently used for such purpose;
peptide sequences identified by the method of the invention, such as: i) the spectrum of peptidyl sequences that are recognized and phosphorylated by a kinase, ii) peptides that include kinase recognition sites and iii) binding entities that specifically distinguish phosphorylated versus non-phosphorylated peptidyl sequences; and
kits for identifying kinase substrates including anti-peptide antibodies for research and diagnostic uses.
Inventors: James S Shaw (NCI)
Fujii K, Zhu G, Liu Y, Hallam J, Chen L, Herrero J, Shaw S. Kinase peptide specificity: improved determination and relevance to protein phosphorylation. Proc Natl Acad Sci USA. 2004 Sep 21;101(38):13744-13749. [ PubMed abs ]
Zhu G, Fujii K, Belkina N, Liu Y, James M, Herrero J, Shaw S. Exceptional disfavor for proline at the P + 1 position among AGC and CAMK kinases establishes reciprocal specificity between them and the proline-directed kinases. J Biol Chem. 2005 Mar 18;280(11):10743-10748. [ PubMed abs ]
Licensing Status: In addition to licensing, the technology is available for further development through collaborative research opportunities with the inventors.
Portfolios: Devices/Instrumentation Devices/Instrumentation - Research Tools and Materials Cancer Cancer - Research Materials Internal Medicine Internal Medicine - Research Materials Research Materials