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Construction of an Infectious Full-Length cDNA Clone of the Porcine Enteric Calicivirus RNA Genome

Posted Feb 13 2013 7:00pm

Description of Invention:
Porcine enteric calicivirus (PEC) is a member of the genus Sapovirus in the family Caliciviridae. This virus causes diarrheal illness in pigs, and is presently the only enteric calicivirus that can be grown in cell culture. In addition to its relevance to veterinary medicine as a diarrheal agent in pigs, PEC serves as an important model for the study of enteric caliciviruses that cause diarrhea and that cannot be grown in cell culture (including the noroviruses represented by Norwalk virus). The development of an infectious cDNA clone is important because it enables the use of “reverse genetics” to engineer mutations of interest into the genome of PEC and to study their effects. In addition, it allows the introduction of foreign coding sequences into the genome of PEC that could be useful for vaccine development in swine and possibly humans. This discovery has both basic research applications such as mapping mutations involved in tissue culture adaptation, tissue tropism, and virulence as well as practical applications such as providing a genetic backbone for the development of chimeric vaccine viruses.

Kyeong-Ok Chang (NIAID)
Lisbeth Kim Y Green (NIAID)
Stanislav V Sosnovtsev (NIAID)
Gael Belliot (NIAID)

Patent Status:
HHS, Reference No. E-214-2003/0

Research Material -- Patent protection is not being pursued for this technology

Related Technologies:
EIR, Reference No. E-198-2003/0
EIR, Reference No. E-212-2003/0
EIR, Reference No. E-283-2003/0

Research Material -- Patent prosecution is not being pursued for these technologies.

Relevant Publication:
  1. The materials are further described in KO Chang et al., "Cell-culture propagation of porcine enteric calicivirus mediated by intestinal contents is dependent on the cyclic AMP signaling pathway," Virology. 2002 Dec 20;304(2):302-310. [ PubMed abs ]

Licensing Status:
The materials embodied in this invention are available nonexclusively through a biological materials license.

Collaborative Research Opportunity:
The Laboratory of Infectious Diseases, NIAID, NIH, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize reagents derived from an infectious cDNA copy of the genome of porcine enteric calicivirus. Please contact Kim Y. Green at for more information.

For Licensing Information Please Contact:
Peter Soukas J.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325 Room 14,
Rockville, MD 20852-3804
United States
Phone: 301-435-4646
Fax: 301-402-0220

Ref No: 909

Updated: 02/2013

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