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Conditionally Immortalized Human Podocyte Cell Lines

Posted Jul 26 2011 8:00pm

Description of Invention:
Podocytes, cells of the visceral epithelium in the kidneys, are a key component of the glomerular filtration barrier. Podocyte damage and loss contribute to the initiation of glomerular diseases. NIH investigators recently established long-term urinary cell cultures from two patients with focal segmental glomerulosclerosis and two healthy volunteers, via transformation with the thermosensitive SV40 large T antigen (U19tsA58) together with human telomerase (hTERT). Characterization of randomly selected clonal cell lines from each human subject showed mRNA expression for the podocyte markers synaptopodin, nestin, and CD2AP in all clones. Podocin mRNA was absent from all clones. The expression of nephrin, Wilms tumor 1 (WT1), and podocalyxin mRNA varied among the clones, which may be due to transformation and/or cloning. These novel human urine-derived podocyte-like epithelial cell lines (HUPECs) generated from urine of patients and healthy volunteers will be useful to study podocyte cell biology.

Applications:
  • Model system for study of glomerular disorders.
  • Useful tools to study podocyte biology.


Advantages:
These podocyte-like cells are unique and novel compared to the currently available podocyte cells because these are obtained from individuals with glomerular disease.

Development Status:
  • Early-stage
  • Pre-clinical
  • In vitro data available


Inventors:
Jeffrey B Kopp (NIDDK)
Toru Sakairi (NIDDK)


Patent Status:
HHS, Reference No. E-252-2010/0

Research Tool — Patent protection is not being pursued for this technology.

Related Technologies:
EIR, Reference No. E-049-2007/0 Research Tool — Model for Study of Glomerular Disorders: Conditionally-Immortalized Mouse Podocyte Cell Line with Tet-on-Regulated Gene Expression (Dr. Jefferey B. Kopp, NIDDK)
EIR, Reference No. E-287-2010/0 Research Tool — CDK4-Transformed Mouse Podocytes Useful for Studying Glomerular Diseases (Drs. Toru Sakairi and Jeffrey B. Kopp, NIDDK)


Relevant Publication:
  1. Sakairi T, et al. [ PMID 19955187 ]



For Licensing Information Please Contact:
Suryanarayana Vepa Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: vepas@mail.nih.gov
Phone: 301-435-5020
Fax: 301-402-0220


Ref No: 2294

Updated: 07/2011

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