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Cell Lines Expressing Nuclear and/or Mitochondrial RNase H1

Posted Nov 05 2012 7:00pm

Description of Invention:
RNase H1 has been shown to remove RNA/DNA hybrids and either too much or too little enzyme can lead to undesirable effects such as deletions of DNA. The gene encoding RNase H1 in mammalian cells produces two forms of the protein. One is targeted to the nucleus of the cell and the other to the mitochondrial organelle. To study the effects of expression as well as to understand the regulation of the frequency with which each form is made, NIH investigators constructed cells derived from HEK293 cells where expression of each or both forms is/are expressed only after addition of doxycycline as a small molecule inducer compound. The set of cell lines could be important in the process of analysis of RNA/DNA hybrids as each cell line expresses different amounts of each form.

Applications:
Research materials to study RNA/DNA hybrids

Advantages:
Not available elsewhere

Development Status:
  • Prototype
  • Pre-clinical
  • In vitro data available


Inventors:
Robert J Crouch (NICHD)
Yutaka Suzuki (NICHD)


Patent Status:
HHS, Reference No. E-273-2012/0

Research Material — Patent protection is not being pursued for this technology.

Relevant Publication:
  1. Suzuki Y, et al. [ PMID 20823270 ]


Collaborative Research Opportunity:
The Program in Genomics of Differentiation, NICHD, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize small molecule inhibitors of RNase H1, genome instability, or transcription and translation. For collaboration opportunities, please contact Joseph Conrad III, Ph.D. at jmconrad@mail.nih.gov .


For Licensing Information Please Contact:
Betty Tong Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: tongb@mail.nih.gov
Phone: 301-594-6565
Fax: 301-402-0220


Ref No: 2490

Updated: 11/2012

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