ATLAS OF IVF EMBRYOS – DAY 2 AND DAY 3 EMBRYOS
DR. SAIPRASAD GUNDETI, SENIOR EMBRYOLOGIST, MALPANI INFERTILITY CLINIC PVT. LTD.

DAY 2 EMBRYOS

• DAY 2 EMBRYOS GENERALLY HAVE 2 TO 4 CELLS IN THEM.
• EMBRYOS WITH 4 CELLS (GRADE A) ARE CONSIDERED AS TOP QUALITY DAY 2 EMBRYOS.

GRADING OF DAY 2 EMBRYOS IS DONE CONSIDERING :

• SIZE OF THE CELLS
• PRESCENCE OF FRAGMENTS / PERCENTAGE OF FRAGMENTATION.

GRADE A EMBRYO

4-CELL GRADE A EMBRYO

• THIS EMBRYO IS A TOP QUALITY DAY 2 EMBRYO
• THIS EMBRYO IS WITH EQUAL SIZED CELLS
• NO FRAGMENTATION IN THE EMBRYO

2-CELL GRADE A EMBRYO

GRADE B EMBRYO

4 CELL GRADE B EMBRYO

• THE EMBRYO IS WITH EQUAL SIZED CELLS
• THE EMBRYO HAS FRAGMENTATION MORE THAN 15 %

GRADE C EMBRYO

4 CELL GRADE C EMBRYO

• THIS EMBRYO IS WITH UNEQUAL SIZED CELLS
• THE EMBRYO HAS FRAGMENTATION MORE THAN 50 %

DAY 3 EMBRYOS

• DAY 3 EMBRYOS GENERALLY HAVE 6 TO 8 CELLS IN THEM.
• EMBRYOS WITH 8 CELLS (GRADE A) ARE CONSIDERED AS TOP QUALITY DAY 3 EMBRYOS.

GRADING OF DAY 3 EMBRYOS IS DONE CONSIDERING :

• SIZE OF THE CELLS
• PRESCENCE OF FRAGMENTS / PERCENTAGE OF FRAGMENTATION.

GRADE A EMBRYO

8-CELL GRADE A EMBRYO

• THIS EMBRYO IS A TOP QUALITY DAY 3 EMBRYO
• THIS EMBRYO IS WITH EQUAL SIZED CELLS
• NO FRAGMENTATION IN THE EMBRYO

6 CELL GRADE A EMBRYO

GRADE B EMBRYO

THIS IS AN 8 CELL GRADE B EMBRYO.

EVEN THOUGH THERE IS NO FRAGMENTATION, THE EMBRYO HAS UNEQUAL SIZED CELLS IN IT, WHICH MAKES IT A GRADE B EMBRYO.

THIS IS AN 8 CELL GRADE B EMBRYO.

THE EMBRYO HAS EQUAL SIZED CELLS IN IT. BUT SINCE IT HAS MORE THAN 15 % FRAGMENTATION IN IT, IT IS A GRADE B EMBRYO.

8-CELL GRADE C EMBRYO

THE EMBRYO IN THE PICTURE IS AN 8 CELL GRADE C EMBRYO.
THE EMBRYO HAS UNEQUAL SIZED CELLS AND FRGMENTATION MORE THAN 50 %

FAST GROWING EMBRYO ON DAY 3

8-CELL GRADE A COMPACTING EMBRYO 12-CELL GRADE A EMBRYO

WE OFTEN SEE SOME EMBRYO GROWING FASTER COMPARED TO OTHER EMBRYOS.
THESE EMBRYO ARE GIVEN PRIORITY WHEN IT COMES TO SELECTING EMBRYOS FOR TRANSFER.