Health knowledge made personal
Join this community!
› Share page:
Search posts:

Anti-H2A Antibody and Method for Detecting DNA Double-Stranded Breaks

Posted Aug 31 2000 5:00pm

Description of Invention:
There presently exist assays for determining DNA breakage due to stresses such as radiation and toxins. These include the TUNEL assay and single cell gel electrophoresis, among others. The difficulty in using these and other assays arises in that a great number of DNA breaks are necessary for adequate detection of the breakage. Since only 40 double-stranded breaks in the DNA leads to cell death, it is evident that there is a need for an assay with greater specificity.

The NIH announces a new technology which relates to such an improvement over current DNA detection assays, with the ability to be sensitive enough to detect a single DNA double-stranded break in a cell's nucleus. This method for detection uses antibodies directed against a synthetic phosphorylated peptide containing the mammalian-H2AX C-terminal sequence for deletion of DNA double-stranded breaks. It centers on the activity of the H2A histone. In response to a DNA break, H2A can become phosphorylated in great numbers and provide protection for the break site to assist in repair. The antibody and method available show specificity for this occurrence and thus allow detection at levels much lower than are presently needed by other detection techniques. Use of such technology could be widespread, both as a diagnostic tool and with specific DNA breakage-related disease and syndrome research.

William M Bonner (NCI)
Efthimia P Rogakou (NCI)

Patent Status:
HHS, Reference No. E-142-1999/0
US, , Patent No. 6,362,317, Issued 26 Mar 2002
US, , Patent No. 6,884,873, Issued 26 Apr 2005
HHS, Reference No. E-142-1999/0

Cancer - Research Materials
Internal Medicine
Internal Medicine - Research Materials

For Additional Information Please Contact:
Tara Kirby Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Phone: 301-435-4426
Fax: 301-402-0220

Ref No: 1240

Updated: 09/2000

Post a comment
Write a comment:

Related Searches