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Two Types of Dentin Sialophosphoprotein (DSPP) Knockout Mice

Posted Jun 15 2010 5:00pm

Description of Invention:
Two types of dentin sialophosphoprotein knockout mice are available for licensing. The technology relates to two separate knockout mouse models of the role of dentin sialophosphoprotein in dentin mineralization and development of teeth. The first knockout mouse is a knockout of the entire DSPP gene, which results in a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposures. DSPP protein in odontoblasts is normally proteolytically cleaved into two products, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). To distinguish the role of the proteolytic fragments, the second knockout mouse (DPPcKO) consists of a transgene expressing the DSP fragment in a DSPP null background. The DPPcKO mouse demonstrates a partial rescue of the DSPP knockout effect and indicates DSP and PPP have distinct roles in dentin development.

Applications:
  • Tool for studying dentin development.
  • Tool for developing treatments for autosomal dominant dentinogenesis imperfecta.


Inventors:
Ashok B Kulkarni (NIDCR)
Shigeki Suzuki (NIDCR)


Patent Status:
HHS, Reference No. E-201-2009/0

Research Tool -- patent protection is not being pursued for this technology

Relevant Publication:
  1. T Sreenath, T Thyagarajan, B Hall, G Longenecker, R D'Souza, S Hong, JT Wright, M MacDougall, J Sauk, AB Kulkarni. Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis imperfecta type III. J Biol Chem. 2003 Jul 4;278(27):24874-24880. [ PubMed abs ]
  2. S Suzuki, T Sreenath, N Haruyama, C Honeycutt, A Terse, A Cho, T Kohler, R Muller, M Goldberg, A Kulkarni. Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. Submitted, 2009.


Licensing Status:
This technology is available as a research tool under a Biological Materials License.


Portfolios:
Dental Technology
Dental Technology - Research Materials
Animal Model



For Additional Information Please Contact:
Steven Standley Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: sstand@mail.nih.gov
Phone: 301-435-4074
Fax: 301-402-0220


Ref No: 2004

Updated: 06/2010

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