North Dakota Another 3F2 Mule Deer Tests Positive for CWD
Posted Nov 23 2011 11:46am
News Release Archives - November 2011
November 22, 2011
Another 3F2 Mule Deer Tests Positive for CWD
A mule deer taken from unit 3F2 during opening weekend of the deer gun season has tested positive for chronic wasting disease.
Dr. Dan Grove, North Dakota Game and Fish Department wildlife veterinarian, said a hunter shot a doe in western Grant County and submitted the head for testing as part of the hunter-harvested surveillance program. Testing was performed at Michigan State University. As of Tuesday, Nov. 22, Game and Fish was awaiting verification of initial tests results from a lab at Iowa State University.
“According to the hunter, the animal looked healthy,” Grove said. “It showed no visible signs of having any health issues.”
This is the third deer to test positive for CWD, and all three were from taken from unit 3F2 in southwestern North Dakota. The first two were during the 2009 and 2010 deer gun seasons. All three were within 15 miles of each other.
“The latest positive emphasizes the importance of continued monitoring along with current and expanding CWD restrictions in and around this unit,” Grove said.
The hunter-harvested surveillance program annually collects samples taken from hunter-harvested deer in specific regions of the state. In addition to unit 3F2, samples during the 2011 deer gun season were collected from units in the central third of the state.
CWD affects the nervous system of members of the deer family and is always fatal. Scientists have found no evidence that CWD can be transmitted naturally to humans or livestock.
Chronic wasting disease in a Wisconsin white-tailed deer farm
Delwyn P. Keane1, Daniel J. Barr, Philip N. Bochsler, S. Mark Hall, Thomas Gidlewski, Katherine I. O'Rourke, Terry R. Spraker and Michael D. Samuel Correspondence: 1Corresponding Author: Delwyn Keane, University of Wisconsin, Wisconsin Veterinary Diagnostic Laboratory, 445 Easterday Lane, Madison, WI 53706. mhtml:%7B33B38F65-8D2E-434D-8F9B-8BDCD77D3066%7Dmid://00000040/!x-usc:mailto:Delwyn.Keane@wvdl.wisc.edu
In September 2002, chronic wasting disease (CWD), a prion disorder of captive and wild cervids, was diagnosed in a white-tailed deer (Odocoileus virginianus) from a captive farm in Wisconsin. The facility was subsequently quarantined, and in January 2006 the remaining 76 deer were depopulated. Sixty animals (79%) were found to be positive by immunohistochemical staining for the abnormal prion protein (PrPCWD) in at least one tissue; the prevalence of positive staining was high even in young deer. Although none of the deer displayed clinical signs suggestive of CWD at depopulation, 49 deer had considerable accumulation of the abnormal prion in the medulla at the level of the obex. Extraneural accumulation of the abnormal protein was observed in 59 deer, with accumulation in the retropharyngeal lymph node in 58 of 59 (98%), in the tonsil in 56 of 59 (95%), and in the rectal mucosal lymphoid tissue in 48 of 58 (83%). The retina was positive in 4 deer, all with marked accumulation of prion in the obex. One deer was considered positive for PrPCWD in the brain but not in the extraneural tissue, a novel observation in white-tailed deer. The infection rate in captive deer was 20-fold higher than in wild deer. Although weakly related to infection rates in extraneural tissues, prion genotype was strongly linked to progression of prion accumulation in the obex. Antemortem testing by biopsy of recto–anal mucosal-associated lymphoid tissue (or other peripheral lymphoid tissue) may be a useful adjunct to tonsil biopsy for surveillance in captive herds at risk for CWD infection.
PLEASE NOTE 76 DEER WERE DEPOPULATED. SIXTY ANIMALS (79%) WERE FOUND TO BE POSITIVE BY IMMUNOHISTOCHEMICAL STAINING FOR THE ABNORMAL PRION PROTEIN (PrPCWD) IN AT LEAST ONE TISSUE; THE PREVALENCE OF POSITIVE STAINING WAS HIGH IN YOUNG DEER. ...TSS
Title: Chronic wasting disease in a Wisconsin captive white-tailed deer farm
Keane, Delwyn - U OF WIS, WIS VET DIAG LA Barr, Daniel - U OF WIS, WIS VET DIAG LA Bochsler, Philip - U OF WIS, WIS VET DIAG LA Hall, S - USDA, APHIS, VS, NVSL Gidlewski, Thomas - USDA, APHIS, VS O`rourke, Katherine Spraker, Terry - CO STATE UNIVERSITY Samuel, Michael - US GEOLOGIC SERVICE
Submitted to: Journal of Veterinary Diagnostic Investigation Publication Type: Peer Reviewed Journal Publication Acceptance Date: May 5, 2008 Publication Date: N/A
Chronic wasting disease is a fatal disease of deer and elk. Clinical signs, including weight loss, frequent urination, excessive thirst, and changes in behavior and gait, have been reported in mule deer and elk with this disorder. Clinical signs in captive white tailed deer are less well understood. In a previous study, a captive facility housed 200 deer, of which half were positive for the disease with no clinical signs reported. In this study, we examined 78 white tailed deer from a captive facility with a history of chronic wasting disease and no animals with clinical signs. Examination of the brain and lymph nodes demonstrated that the abnormal prion protein, a marker for disease, was observed in 60 of the deer. Biopsy of the rectal mucosa, a test that can be performed on live deer, detected 83% of the infected animals. The prion genetics of the deer was strongly linked to the rate of infection and to disease progression. The results demonstrate that clinical signs are a poor indicator of the disease in captive white tailed deer and that routine testing of live deer and comprehensive necropsy surveillance may be needed to identify infected herds. Technical Abstract: Chronic wasting disease CWD is a transmissible spongiform encephalopathy or prion disease of deer and elk in North America. All diseases in this family are characterized by long preclinical incubation periods following by a relatively short clinical course. Endpoint disease is characterized by extensive deposits of aggregates of the abnormal prion protein in the central nervous system,. In deer, the abnormal prion proteins accumulate in some peripheral lymphoid tissues early in disease and are therefore suitable for antemortem and preclinical postmortem diagnostics and for determining disease progression in infected deer. In this study, a herd of deer with previous CWD diagnoses was depopulated. No clinical suspects were identified at that time. Examination of the brain and nodes demonstrated that 79% of the deer were infected. Of the deer with abnormal prion in the peripheral lymphoid system, the retropharyngeal lymph node was the most reliable diagnostic tissue. Biopsy of the rectal mucosal tissue, a site readily sampled in the restrained or chemically immobilized deer, provided an accurate diagnosis in 83% of the infected deer. The retina in the eye of the deer was positive only in late stage cases. This study demonstrated that clinical signs are a poor indicator of disease, supports the use of the retropharyngeal lymph node as the most appropriate postmortem sample, and supports a further evaluation of the rectal mucosal tissue biopsy as an antemortem test on a herd basis.
A. ORAL TRANSMISSION to deer and elk is a very efficient mode for transmission. HIGH PROTEIN FEED will transmit CWD. high protein feed have been fed to deer and elk, especially in game farms.
B. COMMINGLING OF DISEASED ANIMALS AROUND BAIT PILE. chance of the environment in the surrounding areas becoming infected, from feces, urine, shedding, becoming a hot bed for animals to feed, congregate, and become exposed, and there is still very much the possibility of lateral transmission, especially with the CWD TSE, in deer and elk.
WHY ignore sound science ?
IT's the same with pouring a bottle of 100% deer urine scent formulas all over you and the environment, this is a proven mode of transmission. SO WHY DO IT?
leave your egos, and testosterone at the door, and look at the transmission studies, this is NOT rocket science. ...TSS
ENLARGING SPECTRUM OF PRION-LIKE DISEASES Prusiner Colby et al 2011 Prions
David W. Colby1,* and Stanley B. Prusiner1,2
+ Author Affiliations
1Institute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, California 94143 2Department of Neurology, University of California, San Francisco, San Francisco, California 94143 Correspondence: firstname.lastname@example.org
I believe the statement and quote below is incorrect ;
"CWD has been transmitted to cattle after intracerebral inoculation, although the infection rate was low (4 of 13 animals [Hamir et al. 2001]). This finding raised concerns that CWD prions might be transmitted to cattle grazing in contaminated pastures."
Please see ;
Within 26 months post inoculation, 12 inoculated animals had lost weight, revealed abnormal clinical signs, and were euthanatized. Laboratory tests revealed the presence of a unique pattern of the disease agent in tissues of these animals. These findings demonstrate that when CWD is directly inoculated into the brain of cattle, 86% of inoculated cattle develop clinical signs of the disease.
"although the infection rate was low (4 of 13 animals [Hamir et al. 2001])."
shouldn't this be corrected, 86% is NOT a low rate. ...
Terry S. Singeltary Sr. P.O. Box 42 Bacliff, Texas USA 77518
MARCH 1, 2011
UPDATED CORRESPONDENCE FROM AUTHORS OF THIS STUDY I.E. COLBY, PRUSINER ET AL, ABOUT MY CONCERNS OF THE DISCREPANCY BETWEEN THEIR FIGURES AND MY FIGURES OF THE STUDIES ON CWD TRANSMISSION TO CATTLE ;
----- Original Message -----
From: David Colby
Sent: Tuesday, March 01, 2011 8:25 AM
Subject: Re: FW: re-Prions David W. Colby1,* and Stanley B. Prusiner1,2 + Author Affiliations
Dear Terry Singeltary,
Thank you for your correspondence regarding the review article Stanley Prusiner and I recently wrote for Cold Spring Harbor Perspectives. Dr. Prusiner asked that I reply to your message due to his busy schedule. We agree that the transmission of CWD prions to beef livestock would be a troubling development and assessing that risk is important. In our article, we cite a peer-reviewed publication reporting confirmed cases of laboratory transmission based on stringent criteria. The less stringent criteria for transmission described in the abstract you refer to lead to the discrepancy between your numbers and ours and thus the interpretation of the transmission rate. We stand by our assessment of the literature--namely that the transmission rate of CWD to bovines appears relatively low, but we recognize that even a low transmission rate could have important implications for public health and we thank you for bringing attention to this matter.
Warm Regards, David Colby
David Colby, PhDAssistant ProfessorDepartment of Chemical EngineeringUniversity of Delaware