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Cleaning, disinfection and sterilization of surface prion contamination

Posted Sep 25 2013 11:49am
Article in Press

 

Cleaning, disinfection and sterilization of surface prion contamination

 

G. McDonnell Affiliations STERIS Corporation, Mentor, Ohio, USA Corresponding Author InformationCorresponding author. Address: STERIS Corporation, 5960 Heisley Road, Mentor, OH 44060, USA. Tel.: +1 440 392 7118. email address, C. Dehen Affiliations CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA), Fontenay-aux-Roses, France , A. Perrin Affiliations CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA), Fontenay-aux-Roses, France , V. Thomas Affiliations STERIS, CEA, Fontenay-aux-Roses, France , A. Igel-Egalon Affiliations CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA), Fontenay-aux-Roses, France STERIS, CEA, Fontenay-aux-Roses, France , P.A. Burke Affiliations STERIS Corporation, Mentor, Ohio, USA , J.P. Deslys Affiliations CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA), Fontenay-aux-Roses, France , E. Comoy Affiliations CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA), Fontenay-aux-Roses, France

 

Received 3 October 2012; accepted 27 August 2013. published online 25 September 2013. Accepted Manuscript

 

Abstract PDF

 

Summary Background

 

Prion contamination is a risk during device reprocessing, being difficult to remove and inactivate. Little is known of the combined effects of cleaning, disinfection and sterilization during a typical reprocessing cycle in clinical practice.

 

Aim

 

To investigate the combination of cleaning, disinfection and/or sterilization on reducing the risk of surface prion contamination.

 

Methods

 

In vivo test methods were used to study the impact of cleaning alone and cleaning combined with thermal disinfection and high- or low-temperature sterilization processes. A standardized test method, based on contamination of stainless steel wires with high titres of scrapie-infected brain homogenates, was used to determine infectivity reduction.

 

Findings

 

Traditional chemical methods of surface decontamination against prions were confirmed to be effective, but extended steam sterilization was more variable. Steam sterilization alone reduced the risk of prion contamination under normal or extended exposure conditions, but did show significant variation. Thermal disinfection had no impact in these studies. Cleaning with certain defined formulations in combination with steam sterilization can be an effective prion decontamination process, in particular with alkaline formulations. Low-temperature, gaseous hydrogen peroxide sterilization was also confirmed to reduce infectivity in the presence and absence of cleaning.

 

Conclusion

 

Prion decontamination is affected by the full reprocessing cycle used on contaminated surfaces. The correct use of defined cleaning, disinfection and sterilization methods as tested in this report in the scrapie infectivity assay can provide a standard precaution against prion contamination.

 

Keywords: Cleaning, Disinfection, Prion, Reprocessing, Sterilization

 

 


 

 

 

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Iatrogenic Creutzfeldt-Jakob disease via surgical instruments and decontamination possibilities for the TSE prion

 


 

 

 

Friday, August 16, 2013

 

*** Creutzfeldt-Jakob disease (CJD) biannual update August 2013 U.K. and Contaminated blood products induce a highly atypical prion disease devoid of PrPres in primates

 


 

 

Sunday, August 11, 2013

 

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*** Creutzfeldt-Jakob Disease CJD cases rising North America with Canada seeing an extreme increase of 48% between 2008 and 2010

 


 

 

 

Tuesday, September 17, 2013

 

USAHA 116TH ANNUAL MEETING October 18 – 24, 2012 CWD, Scrapie, BSE, TSE prion (September 17, 2013)

 


 

 

 

kind regards, terry
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