Fibroblast

Increased Collagen Synthesis by Scleroderma Skin Fibroblasts In Vitro by Jan Posted Sun 29 Aug 2010 12:00am more rapidly, and fourfold more of its protein synthetic activity is directed to collagen production than in the normal skin fibroblast. Cell-doubling times and morphologic... Increased Collagen Synthesis by Scleroderma Skin Fibroblasts In Vitro A POSSIBLE DEFECT IN THE REGULATION OR ACTIVATION OF THE SCLERODERMA FIBROBLAST A B S T R A C Read on »

Effect of nitric oxide and peroxynitrite on type I collagen synthesis in normal and scleroderma dermal fibroblasts. by Jan Posted Fri 12 Nov 2010 4:19am of ()NO and peroxynitrite (ONOO(-)) on secretion of type I collagen by SSc dermal fibroblasts, compared with those from normal dermal fibroblasts (CON) and a dermal fibroblast cell line (AG). Dermal fibroblasts were incubated with ()NO donors (SNP, DETA-NONOate) with or without the antioxidant ascorbic acid, or ONOO(-) for 24-72 h. In CON and AG fibroblasts, type I collagen Read on »

Scleroderma fibroblasts constitutively express the long pentraxin PTX3 by Jan Posted Sun 13 Mar 2011 12:14pm of PTX3 in normal and scleroderma fibroblasts. Methods. Normal and SSc fibroblasts were cultured in the presence and absence of inflammatory cytokines. PTX3 m R N A expression in fibroblasts was evaluated by Northern analysis. PTX3 protein levels in fibroblast culture medium were estimated by ELISA. R e s u l t s. Normal fibroblasts Read on »

Transcriptional profiling of the scleroderma fibroblast reveals a potential role for connective tissue growth factor (CTGF) in p by Jan Posted Wed 09 Mar 2011 2:20am The cause of fibrotic disease is unknown. We have undertaken transcriptional profiling of dermal fibroblasts cultured from patients with the fibrotic disease scleroderma...), a member of the CCN family of proteins, is overexpressed in SSc fibroblasts. In adult skin, CTGF is not normally expressed in dermal fibroblasts. However, CTGF is induced during Read on »

Modulation of collagen type I, fibronectin and dermal fibroblast function and activity, in systemic sclerosis by the antioxidant by Jan Posted Thu 25 Nov 2010 7:57am Objectives. SSc is characterized by the overproduction of extracellular matrix (ECM) proteins, such as collagen and fibronectin, by activated fibroblasts, as well as oxidative stress. This study investigates the anti-fibrotic potential of the antioxidant epigallocatechin-3-gallate (EGCG) on activated dermal fibroblasts from SSc patients Read on »

Scleroderma Lung Fibroblasts: Contractility and Connective Tissue Growth Factor by Jan Posted Thu 25 Nov 2010 3:06am In the pathogenesis of pulmonary fibrosis in general and systemic sclerosis (SSc, scleroderma) in particular, lung fibroblasts undergo specific phenotypic modulation and develop cytoskeletal features similar to those of smooth muscle cells. These phenotypically altered, activated fibroblasts, or myofibroblasts, express a contractile isoform of actin Read on »

Aminoterminal extension peptides from type I procollagen normalize excessive collagen synthesis of scleroderma fibroblasts by Jan Posted Fri 11 Sep 2009 4:55pm By Thomas Colleague Summary Fibroblasts derived from a skin biopsy of a patient with scleroderma in the sclerotic stage were shown to have a higher rate of DNA synthesis, and to synthesize more collagen than fibroblasts from a healthy control. The addition of procollagen peptides to the culture medium of scleroderma fibroblasts almost normalized the collagen Read on »

Oxidative stress in scleroderma: Maintenance of scleroderma fibroblast phenotype by the constitutive up-regulation of reactive o by Jan Posted Mon 12 Oct 2009 10:02pm By Paola Sambo and colleague Abstract Objective To explore the role of reactive oxygen species (ROS) in the in vitro activation of skin fibroblasts from patients with systemic sclerosis (SSc). Methods Fibroblasts were obtained from involved skin of patients with limited or diffuse SSc. Oxidative activity imaging in living cells was carried Read on »

Chemokine expression by systemic sclerosis fibroblasts: Abnormal regulation of monocyte chemoattractant protein 1 expression by Jan Posted Fri 11 Sep 2009 4:55pm the potential contribution of systemic sclerosis (SSc) fibroblasts to chemokine production and its potential relevance to the pathogenesis of SSc. Methods The expression of messenger RNA (mRNA) for different C-C and C-X-C chemokines by SSc and normal fibroblasts was studied by RNase protection assay. Monocyte chemoattractant protein 1 (MCP-1) protein Read on »

Scleroderma Lung Fibroblasts by Jan Posted Fri 11 Sep 2009 4:56pm well established. These smooth-muscle-like fibroblasts promote deposition of ECM proteins and contractility of lung parenchyma. The present studies were performed to characterize the contractile activity of SSc lung fibroblasts. Previously, we demonstrated that the early stages of interstitial lung disease of SSc are characterized by a prominence of cells Read on »

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